Antibody Name:	Anti-FSP1/S100A4 Antibody [KO Validated]
Antibody SKU:	CAB1631
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human FSP1/S100A4 (NP_002952.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SKOV3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human FSP1/S100A4 (NP_002952.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MACP LEKA LDVM VSTF HKYS GKEG DKFK LNKS ELKE LLTR ELPS FLGK RTDE AAFQ KLMS NLDS NRDN EVDF QEYC VFLS CIAM MCNE FFEG FPDK QPRK K
Gene ID:	6275
Uniprot:	P26447
Cellular Location:
Calculated MW:	11kDa
Observed MW:	12kDa

Synonyms:

18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, S100A4

Background:

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q21 Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus Molecular Function:identical protein binding; protein binding; RAGE receptor binding; calcium ion binding; actin binding; calcium-dependent protein binding Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; epithelial to mesenchymal transition
NCBI Summary:	The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P26447
NCBI GenInfo Identifier:	115601
NCBI Gene ID:	6275
NCBI Accession:	P26447.1
UniProt Secondary Accession:	P26447,Q6ICP8, A8K7R8, D3DV46,
UniProt Related Accession:	P26447
Molecular Weight:	101
NCBI Full Name:	Protein S100-A4
NCBI Synonym Full Names:	S100 calcium binding protein A4
NCBI Official Symbol:	S100A4
NCBI Official Synonym Symbols:	42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
NCBI Protein Information:	protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metas
UniProt Protein Name:	Protein S100-A4
UniProt Synonym Protein Names:	Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4
Protein Family:	Tyrosine-protein kinase
UniProt Gene Name:	S100A4
UniProt Entry Name:	S10A4_HUMAN

	Western blot analysis of extracts of various cell lines, using FSP1/S100A4 antibody (CAB1631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and FSP1/S100A4 knockout (KO) HeLa cells, using FSP1/S100A4 antibody (CAB1631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] FSP1/S100A4 Rabbit pAb (CAB1631) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] FSP1/S100A4 Rabbit pAb (CAB1631) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using FSP1/S100A4 antibody (CAB1631) at dilution of 1:100. Blue: DAPI for nuclear staining.