Name: Anti-FRZB Antibody (CAB6592)
Brand: Developmental Biology
SKU: CAB6592
Availability: OutOfStock

Antibody Name:	Anti-FRZB Antibody
Antibody SKU:	CAB6592
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 166-325 of human FRZB (NP_001454.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 166-325 of human FRZB (NP_001454.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SNGN CRGA SSER CKCK PIRA TQKT YFRN NYNY VIRA KVKE IKTK CHDV TAVV EVKE ILKS SLVN IPRD TVNL YTSS GCLC PPLN VNEE YIIM GYED EERS RLLL VEGS IAEK WKDR LGKK VKRW DMKL RHLG LSKS DSSN SDST QSQK SGRN SNPR QARN
Gene ID:	2487
Uniprot:	Q92765
Cellular Location:	Secreted
Calculated MW:	36kDa
Observed MW:	36kDa

Synonyms:	FRZB, FRE, FRITZ, FRP-3, FRZB-1, FRZB-PEN, FRZB1, FZRB, OS1, SFRP3, SRFP3, hFIZ
Background:	The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility.

UniProt Protein Function:	FRZB: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development. Defects in FRZB are associated with susceptibility to osteoarthritis type 1(OS1). Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Belongs to the secreted frizzled-related protein (sFRP) family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 2q32.1 Cellular Component: extracellular space; membrane; integral to membrane Molecular Function:G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity Biological Process: negative regulation of cell proliferation; convergent extension involved in organogenesis; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; positive regulation of fat cell differentiation; neural crest cell differentiation; negative regulation of cell growth; skeletal development Disease: Osteoarthritis Susceptibility 1
NCBI Summary:	The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. [provided by RefSeq, Apr 2010]
UniProt Code:	Q92765
NCBI GenInfo Identifier:	14194748
NCBI Gene ID:	2487
NCBI Accession:	Q92765.2
UniProt Secondary Accession:	Q92765,O00181, Q99686,
UniProt Related Accession:	Q92765
Molecular Weight:	Calculated: 36kDaObserved: 38kDa
NCBI Full Name:	Secreted frizzled-related protein 3
NCBI Synonym Full Names:	frizzled-related protein
NCBI Official Symbol:	FRZB
NCBI Official Synonym Symbols:	FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
NCBI Protein Information:	secreted frizzled-related protein 3; sFRP-3; frezzled; frizzled homolog-related; frizzled-related protein 1
UniProt Protein Name:	Secreted frizzled-related protein 3
UniProt Synonym Protein Names:	Frezzled; Fritz; Frizzled-related protein 1; FrzB-1
Protein Family:	Frizzy aggregation protein
UniProt Gene Name:	FRZB
UniProt Entry Name:	SFRP3_HUMAN

	Western blot analysis of extracts of mouse skeletal muscle, using FRZB antibody (CAB6592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using FRZB antibody (CAB6592). Blue: DAPI for nuclear staining.