Cell Biology Antibodies 10
Anti-FLOT2 Antibody (CAB6590)
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- SKU:
- CAB6590
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-FLOT2 Antibody |
| Antibody SKU: | CAB6590 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 239-428 of human FLOT2 (NP_004466.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, SKOV3, Mouse heart, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 239-428 of human FLOT2 (NP_004466.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LAYE LQGA REQQ KIRQ EEIE IEVV QRKK QIAV EAQE ILRT DKEL IATV RRPA EAEA HRIQ QIAE GEKV KQVL LAQA EAEK IRKI GEAE AAVI EAMG KAEA ERMK LKAE AYQK YGDA AKMA LVLE ALPQ IAAK IAAP LTKV DEIV VLSG DNSK VTSE VNRL LAEL PASV HALT GVDL SKIP LIKK ATGV QV |
| Gene ID: | 2319 |
| Uniprot: | Q14254 |
| Cellular Location: | Cell membrane, Endosome, Lipid-anchor, Membrane, Peripheral membrane protein, caveola |
| Calculated MW: | 47kDa |
| Observed MW: | 47kDa |
| Synonyms: | FLOT2, ECS-1, ECS1, ESA, ESA1, M17S1 |
| Background: | Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. |
| UniProt Protein Function: | FLOT2: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. Belongs to the band 7/mec-2 family. Flotillin subfamily. |
| UniProt Protein Details: | Protein type:Cell adhesion Chromosomal Location of Human Ortholog: 17q11-q12 Cellular Component: focal adhesion; membrane; endocytic vesicle; perinuclear region of cytoplasm; plasma membrane; caveola; flotillin complex; vesicle; endosome; acrosomal membrane Molecular Function:ionotropic glutamate receptor binding; protein binding; protease binding Biological Process: epidermis development; protein stabilization; cell adhesion |
| NCBI Summary: | Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14254 |
| NCBI GenInfo Identifier: | 254763294 |
| NCBI Gene ID: | 2319 |
| NCBI Accession: | Q14254.2 |
| UniProt Related Accession: | Q14254 |
| Molecular Weight: | ~ 49kDa |
| NCBI Full Name: | Flotillin-2 |
| NCBI Synonym Full Names: | flotillin 2 |
| NCBI Official Symbol: | FLOT2 |
| NCBI Official Synonym Symbols: | ESA; ECS1; ESA1; ECS-1; M17S1 |
| NCBI Protein Information: | flotillin-2 |
| UniProt Protein Name: | Flotillin-2 |
| UniProt Synonym Protein Names: | Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1 |
| Protein Family: | Flotillin |
| UniProt Gene Name: | FLOT2 |
| UniProt Entry Name: | FLOT2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FLOT2 antibody (CAB6590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of MCF7 cells using FLOT2 antibody (CAB6590). Blue: DAPI for nuclear staining. |
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