Developmental Biology
Anti-FLNB Antibody (CAB2481)
- SKU:
- CAB2481
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-FLNB Antibody |
| Antibody SKU: | CAB2481 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3, A-431, Mouse liver, Mouse kidney, Mouse uterus, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PDGT EAEA DVIE NEDG TYDI FYTA AKPG TYVI YVRF GGVD IPNS PFTV MATD GEVT AVEE APVN ACPP GFRP WVTE EAYV PVSD MNGL GFKP FDLV IPFA |
| Gene ID: | 2317 |
| Uniprot: | O75369 |
| Cellular Location: | Cytoplasm, Z line, cell cortex, cytoskeleton, myofibril, sarcomere |
| Calculated MW: | 230-281kDa |
| Observed MW: | 298kDa |
| Synonyms: | FLNB, ABP-278, ABP-280, AOI, FH1, FLN-B, FLN1L, LRS1, SCT, TABP, TAP, filamin-B |
| Background: | This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. |
| UniProt Protein Function: | FLNB: a ubiquitous cytoskeletal protein that links actin filaments to membrane glycoproteins. Six alternatively spliced isoforms have been described. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoforms 1 and 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells, retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord and platelets. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer's disease. Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 3p14.3 Cellular Component: focal adhesion; cytoplasm; integral to membrane; stress fiber; plasma membrane; cell cortex; Z disc; cytosol; actin cytoskeleton Molecular Function:identical protein binding; protein binding; actin binding Biological Process: skeletal muscle development; cytoskeletal anchoring; cytokine and chemokine mediated signaling pathway; actin cytoskeleton organization and biogenesis; signal transduction; cell differentiation Disease: Larsen Syndrome; Boomerang Dysplasia; Atelosteogenesis, Type I; Spondylocarpotarsal Synostosis Syndrome; Atelosteogenesis, Type Iii |
| NCBI Summary: | This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009] |
| UniProt Code: | O75369 |
| NCBI GenInfo Identifier: | 296434507 |
| NCBI Gene ID: | 2317 |
| NCBI Accession: | O75369.2 |
| UniProt Secondary Accession: | O75369,Q13706, Q59EC2, Q60FE7, Q6MZJ1, Q8WXS9, Q8WXT0 B2ZZ83, B2ZZ84, B2ZZ85, C9JKE6, C9JMC4, |
| UniProt Related Accession: | O75369 |
| Molecular Weight: | 2602 |
| NCBI Full Name: | Filamin-B |
| NCBI Synonym Full Names: | filamin B, beta |
| NCBI Official Symbol: | FLNB |
| NCBI Official Synonym Symbols: | AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280 |
| NCBI Protein Information: | filamin-B; filamin-3; beta-filamin; ABP-280 homolog; filamin homolog 1; thyroid autoantigen; actin binding protein 278; actin-binding-like protein; Larsen syndrome 1 (autosomal dominant) |
| UniProt Protein Name: | Filamin-B |
| UniProt Synonym Protein Names: | ABP-278; ABP-280 homolog; Actin-binding-like protein; Beta-filamin; Filamin homolog 1; Fh1; Filamin-3; Thyroid autoantigen; Truncated actin-binding protein; Truncated ABP |
| Protein Family: | Filamin |
| UniProt Gene Name: | FLNB |
| UniProt Entry Name: | FLNB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FLNB antibody (CAB2481) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody (CAB2481) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using FLNB antibody (CAB2481) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody (CAB2481) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using FLNB antibody (CAB2481) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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