Name: Anti-FGR Antibody (CAB2075)
Brand: Immunology Antibodies 2
SKU: CAB2075
Availability: OutOfStock

Antibody Name:	Anti-FGR Antibody
Antibody SKU:	CAB2075
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FGR (NP_005239.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HL-60, U-937, Raji

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FGR (NP_005239.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGCV FCKK LEPV ATAK EDAG LEGD FRSY GAAD HYGP DPTK ARPA SSFA HIPN YSNF SSQA INPG FLDS GTIR GVSG IGVT LFIA LYDY EART EDDL TFTK
Gene ID:	2268
Uniprot:	P09769
Cellular Location:	Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Lipid-anchor, Mitochondrion inner membrane, Mitochondrion intermembrane space, Peripheral membrane protein, cytoskeleton, cytosol, ruffle membrane
Calculated MW:	59kDa
Observed MW:	53kDa

Synonyms:

FGR, SRC2, c-fgr, c-src2, p55-Fgr, p55c-fgr, p58-Fgr, p58c-fgr

Background:

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	FGR: a non-receptor tyrosine kinase of the Src family. Contains one SH2 and one SH3 domain. Beta 2 integrin signaling induces its c-Cbl-mediated ubiquitination in adherent neutrophils. An ortholog of v-fgr feline oncogene. Mildly amplified in hormone-resistant prostate cancer. Copy number either increased or decreased in other cancers.
UniProt Protein Details:	Protein type:Protein kinase, TK; EC 2.7.10.2; Mitochondrial; Kinase, protein; Oncoprotein; Protein kinase, tyrosine (non-receptor); TK group; Src family Chromosomal Location of Human Ortholog: 1p36.2-p36.1 Cellular Component: actin cytoskeleton; cytoskeleton; cytosol; extrinsic to internal side of plasma membrane; mitochondrial inner membrane; mitochondrial intermembrane space; plasma membrane Molecular Function:ATP binding; non-membrane spanning protein tyrosine kinase activity; phosphotyrosine binding; protein binding; protein kinase binding; protein-tyrosine kinase activity Biological Process: cell differentiation; cell migration; defense response to Gram-positive bacterium; immune response-regulating cell surface receptor signaling pathway; innate immune response; integrin-mediated signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of cell migration; positive regulation of cytokine secretion; positive regulation of mast cell degranulation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cell shape; regulation of innate immune response; regulation of phagocytosis; regulation of protein kinase activity; response to virus; transmembrane receptor protein tyrosine kinase signaling pathway
NCBI Summary:	This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P09769
NCBI GenInfo Identifier:	125358
NCBI Gene ID:	2268
NCBI Accession:	P09769.2
UniProt Secondary Accession:	P09769,Q9UIQ3, D3DPL7,
UniProt Related Accession:	P09769
Molecular Weight:	59,479 Da
NCBI Full Name:	Tyrosine-protein kinase Fgr
NCBI Synonym Full Names:	FGR proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol:	FGR
NCBI Official Synonym Symbols:	SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
NCBI Protein Information:	tyrosine-protein kinase Fgr
UniProt Protein Name:	Tyrosine-protein kinase Fgr
UniProt Synonym Protein Names:	Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog; Proto-oncogene c-Fgr; p55-Fgr; p58-Fgr; p58c-Fgr
Protein Family:	Tyrosine-protein kinase
UniProt Gene Name:	FGR
UniProt Entry Name:	FGR_HUMAN

Western blot analysis of extracts of various cell lines, using FGR antibody (CAB2075) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Antibodies

FGR Antibody (PACO00590)

FGR Antibody (PACO02722)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)