Cell Biology Antibodies 7
Anti-FGD1 Antibody (CAB16374)
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- SKU:
- CAB16374
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-FGD1 Antibody |
| Antibody SKU: | CAB16374 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FGD1 (NP_004454.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, BxPC-3, HepG2, U2OS |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FGD1 (NP_004454.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LLNS TNRE DEDT PPNS PNVD LGKR APTP IREK EVTM CMRC QEPF NSIT KRRH HCKA CGHV VCGK CSEF RARL VYDN NRSN RVCT DCYV ALHG VPGS SPAC S |
| Gene ID: | 2245 |
| Uniprot: | P98174 |
| Cellular Location: | Cell projection, Cytoplasm, cytoskeleton, lamellipodium, ruffle |
| Calculated MW: | 106kDa |
| Observed MW: | 110kDa |
| Synonyms: | FGD1, AAS, FGDY, MRXS16, ZFYVE3, FYVE |
| Background: | This gene encodes a protein that contains Dbl (DH) and pleckstrin (PH) homology domains and is similar to the Rho family of small GTP-binding proteins. The encoded protein specifically binds to the Rho family GTPase Cdc42Hs and can stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. Defects in this gene are the cause of faciogenital dysplasia and X-linked mental retardation, syndromatic 16. |
| NCBI Summary: | This gene encodes a protein that contains Dbl (DH) and pleckstrin (PH) homology domains and is similar to the Rho family of small GTP-binding proteins. The encoded protein specifically binds to the Rho family GTPase Cdc42Hs and can stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. Defects in this gene are the cause of the faciogenital dysplasia in Aarskog-Scott syndrome and a syndromatic form of X-linked cognitive disability. [provided by RefSeq, Jul 2017] |
| UniProt Code: | P98174 |
| NCBI GenInfo Identifier: | 24797153 |
| NCBI Gene ID: | 2245 |
| NCBI Accession: | NP_004454 |
| UniProt Related Accession: | P98174 |
| Molecular Weight: | 106kDa |
| NCBI Full Name: | FYVE, RhoGEF and PH domain-containing protein 1 |
| NCBI Synonym Full Names: | FYVE, RhoGEF and PH domain containing 1 |
| NCBI Official Symbol: | FGD1 |
| NCBI Official Synonym Symbols: | AAS; FGDY; MRXS16; ZFYVE3 |
| NCBI Protein Information: | FYVE, RhoGEF and PH domain-containing protein 1 |
| UniProt Protein Name: | FYVE, RhoGEF and PH domain-containing protein 1 |
| UniProt Synonym Protein Names: | Faciogenital dysplasia 1 protein; Rho/Rac guanine nucleotide exchange factor FGD1; Rho/Rac GEF; Zinc finger FYVE domain-containing protein 3 |
| Protein Family: | FYVE, RhoGEF and PH domain-containing protein |
| UniProt Gene Name: | FGD1 |
| UniProt Entry Name: | FGD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FGD1 antibody (CAB16374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using FGD1 antibody (CAB16374) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using FGD1 antibody (CAB16374) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using FGD1 antibody (CAB16374) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using FGD1 Polyclonal Antibody (CAB16374) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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