Antibody Name:	Anti-FEN1 Antibody
Antibody SKU:	CAB1175
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN1 (NP_004102.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	NIH/3T3, HeLa, Jurkat, Y79, Mouse spleen, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN1 (NP_004102.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GDVL QNEE GETT SHLM GMFY RTIR MMEN GIKP VYVF DGKP PQLK SGEL AKRS ERRA EAEK QLQQ AQAA GAEQ EVEK FTKR LVKV TKQH NDEC KHLL SLMG IPYL DAPS EAEA SCAA LVKA GKVY AAAT EDMD CLTF GSPV LMRH LTAS EAKK LPIQ EFHL SRIL QELG LNQE QFVD LCIL LGSD YCES IRGI GPKR AVDL IQKH KSIE EIVR RLDP NKYP VPEN WLHK EAHQ LFLE PEVL DPES VELK WSEP NEEE LIKF MCGE KQFS EERI RSGV KRLS KSRQ GSTQ GRLD DFFK VTGS LSSA KRKE PEPK GSTK KKAK TGAA GKFK RGK
Gene ID:	2237
Uniprot:	P39748
Cellular Location:	Mitochondrion, Nucleus, nucleolus, nucleoplasm
Calculated MW:	35kDa/42kDa
Observed MW:	48kDa

Synonyms:

FEN1, FEN-1, MF1, RAD2

Background:

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

UniProt Protein Function:	FEN1: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.
UniProt Protein Details:	Protein type:Nucleolus; EC 3.1.-.-; Nuclear receptor co-regulator; DNA repair, damage; Ribonuclease; DNA-binding; Deoxyribonuclease Chromosomal Location of Human Ortholog: 11q12 Cellular Component: membrane; mitochondrion; nuclear chromosome, telomeric region; nucleolus; nucleoplasm; nucleus; plasma membrane; protein complex Molecular Function:5'-3' exonuclease activity; 5'-flap endonuclease activity; damaged DNA binding; DNA binding; double-stranded DNA binding; double-stranded DNA specific exodeoxyribonuclease activity; endonuclease activity; exonuclease activity; protein binding; ribonuclease H activity Biological Process: DNA repair; DNA replication; DNA replication, removal of RNA primer; double-strand break repair; double-strand break repair via homologous recombination; telomere maintenance via recombination; UV protection
NCBI Summary:	The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]
UniProt Code:	P39748
NCBI GenInfo Identifier:	729475
NCBI Gene ID:	2237
NCBI Accession:	P39748.1
UniProt Related Accession:	P39748,AAB32522
Molecular Weight:	35,673 Da
NCBI Full Name:	Flap endonuclease 1
NCBI Synonym Full Names:	flap structure-specific endonuclease 1
NCBI Official Symbol:	FEN1
NCBI Official Synonym Symbols:	MF1; RAD2; FEN-1
NCBI Protein Information:	flap endonuclease 1
UniProt Protein Name:	Flap endonuclease 1
UniProt Synonym Protein Names:	DNase IV; Flap structure-specific endonuclease 1
UniProt Gene Name:	FEN1
UniProt Entry Name:	FEN1_HUMAN

	Western blot analysis of extracts of various cell lines, using FEN1 antibody (CAB1175) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human lung cancer using FEN1 antibody (CAB1175) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using FEN1 antibody (CAB1175) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using FEN1 antibody (CAB1175).
	Immunofluorescence analysis of HeLa cells using FEN1 antibody (CAB1175).