Antibody Name:	Anti-FDPS Antibody
Antibody SKU:	CAB5744
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-419 of human FDPS (NP_001129293.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HepG2, HeLa, MCF7, U-251MG, BxPC-3, Mouse liver, Mouse ovary, Rat liver, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-419 of human FDPS (NP_001129293.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IGGK YNRG LTVV VAFR ELVE PRKQ DADS LQRA WTVG WCVE LLQA FFLV ADDI MDSS LTRR GQIC WYQK PGVG LDAI NDAN LLEA CIYR LLKL YCRE QPYY LNLI ELFL QSSY QTEI GQTL DLLT APQG NVDL VRFT EKRY KSIV KYKT AFYS FYLP IAAA MYMA GIDG EKEH ANAK KILL EMGE FFQI QDDY LDLF GDPS VTGK IGTD IQDN KCSW LVVQ CLQR ATPE QYQI LKEN YGQK EAEK VARV KALY EELD LPAV FLQY EEDS YSHI MALI EQYA APLP PAVF LGLA RKIY KRRK
Gene ID:	2224
Uniprot:	P14324
Cellular Location:	Cytoplasm
Calculated MW:	40kDa/48kDa
Observed MW:	38kDa

Synonyms:

FDPS, FPPS, FPS, POROK9

Background:

This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	FDPS: Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate. Belongs to the FPP/GGPP synthase family.
UniProt Protein Details:	Protein type:EC 2.5.1.10; Transferase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; EC 2.5.1.1; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q22 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol Molecular Function:geranyltranstransferase activity; dimethylallyltranstransferase activity; metal ion binding Biological Process: viral reproduction; geranyl diphosphate biosynthetic process; farnesyl diphosphate biosynthetic process; cholesterol biosynthetic process
NCBI Summary:	This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2008]
UniProt Code:	P14324
NCBI GenInfo Identifier:	215274250
NCBI Gene ID:	2224
NCBI Accession:	P14324.4
UniProt Secondary Accession:	P14324,Q96G29, D3DV91, E9PCI9,
UniProt Related Accession:	P14324
Molecular Weight:	40,532 Da
NCBI Full Name:	Farnesyl pyrophosphate synthase
NCBI Synonym Full Names:	farnesyl diphosphate synthase
NCBI Official Symbol:	FDPS
NCBI Official Synonym Symbols:	FPS; FPPS
NCBI Protein Information:	farnesyl pyrophosphate synthase; FPP synthase; FPP synthetase; geranyltranstransferase; dimethylallyltranstransferase; (2E,6E)-farnesyl diphosphate synthase; farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase
UniProt Protein Name:	Farnesyl pyrophosphate synthase
UniProt Synonym Protein Names:	(2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase (EC:2.5.1.1); Farnesyl diphosphate synthase; Geranyltranstransferase
Protein Family:	Farnesyl diphosphate synthase
UniProt Gene Name:	FDPS
UniProt Entry Name:	FPPS_HUMAN

	Western blot analysis of extracts of various cell lines, using FDPS antibody (CAB5744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using FDPS antibody (CAB5744). Blue: DAPI for nuclear staining.