Immunology Antibodies 2
Anti-FCN1 Antibody (CAB6587)
- SKU:
- CAB6587
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-FCN1 Antibody |
| Antibody SKU: | CAB6587 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-326 of human FCN1 (NP_001994.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-937, U-251MG, HepG2, Mouse liver, Mouse heart, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-326 of human FCN1 (NP_001994.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ADTC PEVK VVGL EGSD KLTI LRGC PGLP GAPG PKGE AGVI GERG ERGL PGAP GKAG PVGP KGDR GEKG MRGE KGDA GQSQ SCAT GPRN CKDL LDRG YFLS GWHT IYLP DCRP LTVL CDMD TDGG GWTV FQRR MDGS VDFY RDWA AYKQ GFGS QLGE FWLG NDNI HALT AQGS SELR VDLV DFEG NHQF AKYK SFKV ADEA EKYK LVLG AFVG GSAG NSLT GHNN NFFS TKDQ DNDV SSSN CAEK FQGA WWYA DCHA SNLN GLYL MGPH ESYA NGIN WSAA KGYK YSYK VSEM KVRP A |
| Gene ID: | 2219 |
| Uniprot: | O00602 |
| Cellular Location: | Cell membrane, Extracellular side, Peripheral membrane protein, Secreted |
| Calculated MW: | 35kDa |
| Observed MW: | 38kDa |
| Synonyms: | FCN1, FCNM, ficolin-1 |
| Background: | The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. |
| UniProt Protein Function: | FCN1: Complement-activating lectin and pattern recognition receptor. Binds GlcNAc. Binds preferentially to 9-O-acetylated 2- 6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. Belongs to the ficolin lectin family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 9q34 Cellular Component: collagen; extrinsic to external side of plasma membrane; extracellular region Molecular Function:protein binding; G-protein-coupled receptor binding; metal ion binding; carbohydrate binding; pattern recognition receptor activity; sialic acid binding Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of virion penetration into host cell; cell surface pattern recognition receptor signaling pathway; innate immune response; complement activation, lectin pathway; recognition of apoptotic cell; complement activation |
| NCBI Summary: | The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00602 |
| NCBI GenInfo Identifier: | 20455484 |
| NCBI Gene ID: | 2219 |
| NCBI Accession: | O00602.2 |
| UniProt Secondary Accession: | O00602,Q5VYV5, Q92596, |
| UniProt Related Accession: | O00602 |
| Molecular Weight: | 35,078 Da |
| NCBI Full Name: | Ficolin-1 |
| NCBI Synonym Full Names: | ficolin (collagen/fibrinogen domain containing) 1 |
| NCBI Official Symbol: | FCN1 |
| NCBI Official Synonym Symbols: | FCNM |
| NCBI Protein Information: | ficolin-1; M-ficolin; ficolin-A; ficolin-alpha; collagen/fibrinogen domain-containing protein 1; ficolin (collagen/fibrinogen domain-containing) 1 |
| UniProt Protein Name: | Ficolin-1 |
| UniProt Synonym Protein Names: | Collagen/fibrinogen domain-containing protein 1; Ficolin-A; Ficolin-alpha; M-ficolin |
| Protein Family: | Ficolin |
| UniProt Gene Name: | FCN1 |
| UniProt Entry Name: | FCN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FCN1 antibody (CAB6587) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human tonsil using FCN1 Antibody (CAB6587) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human brain astrocytoma using FCN1 Antibody (CAB6587) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using FCN1 Antibody (CAB6587) at dilution of 1:100 (40x lens). |
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