Signal Transduction Antibodies 3

Anti-FBXW4 Antibody (CAB8149)

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SKU:
CAB8149
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:FBXW4 Rabbit Polyclonal Antibody
Antibody SKU:CAB8149
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 143-412 of human FBXW4 (NP_071322.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 143-412 of human FBXW4 (NP_071322.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QFRP DGAS LNRR PLGV FAGH DEDV CHFV LANS HIVS AGGD GKIG IHKI HSTF TVKY SAHE QEVN CVDC KGGI IVSG SRDR TAKV WPLA SGRL GQCL HTIQ TEDR VWSI AISP LLSS FVTG TACC GHFS PLRI WDLN SGQL MTHL GSDF PPGA GVLD VMYE SPFT LLSC GYDT YVRY WDLR TSVR KCVM EWEE PHDS TLYC LQTD GNHL LATG SSYY GVVR LWDR RQRA CLHA FPLT STPL SSPV YCLR LTTK HLYA ALSY NLHV LDFQ NP
Gene ID:6468
Uniprot:P57775
Cellular Location:
Calculated MW:46kDa
Observed MW:46kDa
Synonyms:FBXW4, DAC, FBW4, FBWD4, SHFM3, SHSF3
Background:This gene is a member of the F-box/WD-40 gene family, which recruit specific target proteins through their WD-40 protein-protein binding domains for ubiquitin mediated degradation. In mouse, a highly similar protein is thought to be responsible for maintaining the apical ectodermal ridge of developing limb buds; disruption of the mouse gene results in the absence of central digits, underdeveloped or absent metacarpal/metatarsal bones and syndactyly. This phenotype is remarkably similar to split hand-split foot malformation in humans, a clinically heterogeneous condition with a variety of modes of transmission. An autosomal recessive form has been mapped to the chromosomal region where this gene is located, and complex rearrangements involving duplications of this gene and others have been associated with the condition. A pseudogene of this locus has been mapped to one of the introns of the BCR gene on chromosome 22.
UniProt Protein Function:FBXW4: Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling. Defects in FBXW4 are a cause of split-hand/foot malformation type 3 (SHFM3). SHFM3 is an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.
UniProt Protein Details:

Protein type:Cell development/differentiation; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: ubiquitin ligase complex

Biological Process: ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway; cartilage development; positive regulation of mesenchymal cell proliferation; embryonic digit morphogenesis; embryonic limb morphogenesis

Disease: Split-hand/foot Malformation 3

NCBI Summary:This gene is a member of the F-box/WD-40 gene family, which recruit specific target proteins through their WD-40 protein-protein binding domains for ubiquitin mediated degradation. In mouse, a highly similar protein is thought to be responsible for maintaining the apical ectodermal ridge of developing limb buds; disruption of the mouse gene results in the absence of central digits, underdeveloped or absent metacarpal/metatarsal bones and syndactyly. This phenotype is remarkably similar to split hand-split foot malformation in humans, a clinically heterogeneous condition with a variety of modes of transmission. An autosomal recessive form has been mapped to the chromosomal region where this gene is located, and complex rearrangements involving duplications of this gene and others have been associated with the condition. A pseudogene of this locus has been mapped to one of the introns of the BCR gene on chromosome 22. [provided by RefSeq, Jul 2008]
UniProt Code:P57775
NCBI GenInfo Identifier:11545739
NCBI Gene ID:6468
NCBI Accession:BC007380
UniProt Secondary Accession:P57775,Q5SVS1, Q96IM6,
UniProt Related Accession:P57775
Molecular Weight:Calculated MW 46 kDaObserved MW 50 kDa
NCBI Full Name:F-box/WD repeat-containing protein 4
NCBI Synonym Full Names:F-box and WD repeat domain containing 4
NCBI Official Symbol:FBXW4  
NCBI Official Synonym Symbols:DAC; FBW4; FBWD4; SHFM3; SHSF3  
NCBI Protein Information:F-box/WD repeat-containing protein 4; F-box and WD-40 domain protein 4; F-box and WD-40 domain-containing protein 4; F-box/WD repeat protein 4; dactylin
UniProt Protein Name:F-box/WD repeat-containing protein 4
UniProt Synonym Protein Names:Dactylin; F-box and WD-40 domain-containing protein 4
Protein Family:F-box/WD repeat-containing protein
UniProt Gene Name:FBXW4  
UniProt Entry Name:FBXW4_HUMAN
Anti-FBXW4 Polyclonal Antibody (CAB8149)Western blot analysis of extracts of mouse brain, using FBXW4 antibody (CAB8149) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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