Signal Transduction Antibodies 2
Anti-FBXO3 Antibody (CAB15813)
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- SKU:
- CAB15813
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-FBXO3 Antibody |
| Antibody SKU: | CAB15813 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human FBXO3 (NP_036307.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse liver, Mouse lung, Mouse eye |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human FBXO3 (NP_036307.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAM ETET APLT LESL PTDP LLLI LSFL DYRD LINC CYVS RRLS QLSS HDPL WRRH CKKY WLIS EEEK TQKN QCWK SLFI DTYS DVGR YIDH YAAI KKAW DDLK KYLE PRCP RMVL SLKE GARE EDLD AVEA QIGC KLPD DYRC SYRI HNGQ KLVV PGLL GSMA LSNH YRSE DLLD VDTA AGGF QQRQ GLKY CLPL TFCI HTGL SQYI AVEA AEGR NKNE VFYQ CPDQ MARN PAAI DMFI |
| Gene ID: | 26273 |
| Uniprot: | Q9UK99 |
| Cellular Location: | Nucleus |
| Calculated MW: | 46kDa/47kDa/54kDa |
| Observed MW: | 54kDa |
| Synonyms: | FBXO3, FBA, FBX3 |
| Background: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end. |
| UniProt Protein Function: | Function: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Ref.6 |
| UniProt Protein Details: | Subunit structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex. Ref.6 Subcellular location: Nucleus. Note: Colocalizes with PML at the peripheries of nuclear bodies. Sequence similarities: Contains 1 apaG domain.Contains 1 F-box domain. |
| NCBI Summary: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UK99 |
| NCBI GenInfo Identifier: | 71297179 |
| NCBI Gene ID: | 26273 |
| NCBI Accession: | AAH39291.1 |
| UniProt Secondary Accession: | Q9UK99,Q86X90, Q9H0V2, Q9NUX2, B3KY16, D3DR05, |
| UniProt Related Accession: | Q9UK99 |
| Molecular Weight: | 54,561 Da |
| NCBI Full Name: | FBXO3 protein |
| NCBI Synonym Full Names: | F-box protein 3 |
| NCBI Official Symbol: | FBXO3 |
| NCBI Official Synonym Symbols: | FBA; FBX3 |
| NCBI Protein Information: | F-box only protein 3; F-box protein FBX3 |
| UniProt Protein Name: | F-box only protein 3 |
| Protein Family: | F-box only protein |
| UniProt Gene Name: | FBXO3 |
| UniProt Entry Name: | FBX3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using FBXO3 Rabbit pAb (CAB15813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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