Antibody Name:	Anti-FANCL Antibody
Antibody SKU:	CAB6812
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human FANCL (NP_060532.2).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:20 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human FANCL (NP_060532.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAVT EASL LRQC PLLL PQNR SKTV YEGF ISAQ GRDF HLRI VLPE DLQL KNAR LLCS WQLR TILS GYHR IVQQ RMQH SPDL MSFM MELK MLLE VALK NRQE LYAL PPPP QFYS SLIE EIGT LGWD KLVY ADTC FSTI KLKA EDAS GREH LITL KLKA KYPA ESPD YFVD FPVP FCAS WTPQ SSLI SIYS QFLA AIES LKAF WDVM DEID EKTW VLEP EKPP RSAT ARRI ALGN NVSI NIEV DPRH PTML PECF FLG
Gene ID:	55120
Uniprot:	Q9NW38
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	42kDa/43kDa
Observed MW:	38kDa

Synonyms:

FANCL, FAAP43, PHF9, POG

Background:

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group L. Alternative splicing results in two transcript variants encoding different isoforms.

UniProt Protein Function:	FANCL: Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth. Defects in FANCL are the cause of Fanconi anemia complementation group L (FANCL). FANCL is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; EC 6.3.2.19; Ligase Chromosomal Location of Human Ortholog: 2p16.1 Cellular Component: nucleoplasm; cytoplasm; nuclear envelope Molecular Function:zinc ion binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ligase activity Biological Process: protein monoubiquitination; gamete generation; DNA repair; response to DNA damage stimulus; regulation of cell proliferation Disease: Fanconi Anemia, Complementation Group L; Tracheoesophageal Fistula With Or Without Esophageal Atresia
NCBI Summary:	The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group L. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NW38
NCBI GenInfo Identifier:	116241360
NCBI Gene ID:	55120
NCBI Accession:	Q9NW38.2
UniProt Secondary Accession:	Q9NW38,Q6GU60,
UniProt Related Accession:	Q9NW38
Molecular Weight:	375
NCBI Full Name:	E3 ubiquitin-protein ligase FANCL
NCBI Synonym Full Names:	Fanconi anemia, complementation group L
NCBI Official Symbol:	FANCL
NCBI Official Synonym Symbols:	POG; PHF9; FAAP43
NCBI Protein Information:	E3 ubiquitin-protein ligase FANCL; PHD finger protein 9; fanconi anemia group L protein; fanconi anemia-associated polypeptide of 43 kDa
UniProt Protein Name:	E3 ubiquitin-protein ligase FANCL
UniProt Synonym Protein Names:	Fanconi anemia group L protein; Fanconi anemia-associated polypeptide of 43 kDa; FAAP43
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	FANCL
UniProt Entry Name:	FANCL_HUMAN

	Western blot analysis of extracts of mouse thymus, using FANCL antibody (CAB6812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse stomach using FANCL antibody (CAB6812) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophagus using FANCL antibody (CAB6812) at dilution of 1:100 (40x lens).