Epigenetics & Nuclear Signaling Antibodies 2

Anti-EZH1 Antibody (CAB5818)

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SKU:
CAB5818
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-EZH1 Antibody
Antibody SKU:CAB5818
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-280 of human EZH1 (NP_001982.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-280 of human EZH1 (NP_001982.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GEEE MIPG SVLI SDAV FLEL VDAL NQYS DEEE EGHN DTSD GKQD DSKE DLPV TRKR KRHA IEGN KKSS KKQF PNDM IFSA IASM FPEN GVPD DMKE RYRE LTEM SDPN ALPP QCTP NIDG P
Gene ID:2145
Uniprot:Q92800
Cellular Location:Nucleus
Calculated MW:68kDa/76kDa/80kDa/85kDa/86kDa
Observed MW:85kDa
Synonyms:EZH1, KMT6B
Background:EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]
UniProt Protein Function:EZH1: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Belongs to the histone-lysine methyltransferase family. EZ subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 17q21.1-q21.3

Cellular Component: nucleoplasm; ESC/E(Z) complex

Molecular Function:chromatin binding; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: anatomical structure morphogenesis; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter

NCBI Summary:EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]
UniProt Code:Q92800
NCBI GenInfo Identifier:3334182
NCBI Gene ID:2145
NCBI Accession:Q92800.2
UniProt Secondary Accession:Q92800,O43287, Q14459, Q53XP3, A6NCH6, B4DIJ1, B4DIZ7 B4DSS2, B4E3R7,
UniProt Related Accession:Q92800
Molecular Weight:747
NCBI Full Name:Histone-lysine N-methyltransferase EZH1
NCBI Synonym Full Names:enhancer of zeste 1 polycomb repressive complex 2 subunit
NCBI Official Symbol:EZH1  
NCBI Official Synonym Symbols:KMT6B  
NCBI Protein Information:histone-lysine N-methyltransferase EZH1; ENX-2; enhancer of zeste homolog 1
UniProt Protein Name:Histone-lysine N-methyltransferase EZH1
UniProt Synonym Protein Names:ENX-2; Enhancer of zeste homolog 1
Protein Family:Histone-lysine N-methyltransferase
UniProt Gene Name:EZH1  
UniProt Entry Name:EZH1_HUMAN
Anti-EZH1 Antibody (CAB5818)Western blot analysis of extracts of mouse lung, using EZH1 antibody (CAB5818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-EZH1 Antibody (CAB5818)Immunofluorescence analysis of A549 cells using EZH1 antibody (CAB5818). Blue: DAPI for nuclear staining.
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