Name: Anti-EXOSC4 Antibody (CAB9147)
Brand: Epigenetics & Nuclear Signaling Antibodies 4
SKU: CAB9147
Availability: OutOfStock

Antibody Name:	Anti-EXOSC4 Antibody
Antibody SKU:	CAB9147
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EXOSC4 (NP_061910.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HeLa, A-431, Jurkat, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EXOSC4 (NP_061910.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGL ELLS DQGY RVDG RRAG ELRK IQAR MGVF AQAD GSAY IEQG NTKA LAVV YGPH EIRG SRAR ALPD RALV NCQY SSAT FSTG ERKR RPHG DRKS CEMG LQLR QTFE AAIL TQLH PRSQ IDIY VQVL QADG GTYA ACVN AATL AVLD AGIP MRDF VCAC SAGF VDGT ALAD LSHV EEAA GGPQ LALA LLPA SGQI ALLE MDAR LHED HLER VLEA AAQA ARDV HTLL DRVV RQHV REAS ILLG D
Gene ID:	54512
Uniprot:	Q9NPD3
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	26kDa
Observed MW:	26kDa

Synonyms:	EXOSC4, RRP41, RRP41A, Rrp41p, SKI6, Ski6p, hRrp41p, p12A
Background:

UniProt Protein Function:	EXOSC4: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC4 binds to ARE-containing RNAs. Belongs to the RNase PH family.
UniProt Protein Details:	Protein type:Ribonuclease; Nucleolus; EC 3.1.13.- Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytoplasm; cytosol; exosome (RNase complex); nuclear exosome (RNase complex); nucleolus; nucleoplasm; nucleus Molecular Function:AU-rich element binding; exoribonuclease activity; protein binding Biological Process: defense response to virus; DNA deamination; maturation of 5.8S rRNA; positive regulation of cell growth; regulation of mRNA stability; rRNA 3'-end processing; rRNA catabolic process; rRNA processing
UniProt Code:	Q9NPD3
NCBI GenInfo Identifier:	14285756
NCBI Gene ID:	54512
NCBI Accession:	Q9NPD3.3
UniProt Related Accession:	Q9NPD3
Molecular Weight:	26,383 Da
NCBI Full Name:	Exosome complex component RRP41
NCBI Synonym Full Names:	exosome component 4
NCBI Official Symbol:	EXOSC4
NCBI Official Synonym Symbols:	SKI6; p12A; RRP41; Ski6p; RRP41A; Rrp41p; hRrp41p
NCBI Protein Information:	exosome complex component RRP41
UniProt Protein Name:	Exosome complex component RRP41
UniProt Synonym Protein Names:	Exosome component 4; Ribosomal RNA-processing protein 41; p12A
UniProt Gene Name:	EXOSC4
UniProt Entry Name:	EXOS4_HUMAN

Anti-EXOSC4 Polyclonal Antibody (CAB9147)

Western blot analysis of extracts of various cell lines, using EXOSC4 antibody (CAB9147) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

EXOSC4 Antibody (PACO09171)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)