Antibody Name:	EXOC2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8235
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human EXOC2 (NP_060773.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, MCF7, HeLa, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human EXOC2 (NP_060773.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSRS RQPP LVTG ISPN EGIP WTKV TIRG ENLG TGPT DLIG LTIC GHNC LLTA EWMS ASKI VCRV GQAK NDKG DIIV TTKS GGRG TSTV SFKL LKPE KIGI LDQS AVWV DEMN YYDM RTDR NKGI PPLS LRPA NPLG IEIE KSKF SQKD LEML FHGM SADF TSEN FSAA WYLI ENHS NTSF EQLK MAVT NLKR QANK KSEG SLAY VKGG LSTF FEAQ DALS
Gene ID:	55770
Uniprot:	Q96KP1
Cellular Location:
Calculated MW:	104kDa
Observed MW:	108kDa

Synonyms:

EXOC2, SEC5, SEC5L1, Sec5p

Background:

The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	Sec5: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. Belongs to the SEC5 family.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 6p25.3 Cellular Component: membrane; intracellular membrane-bound organelle; exocyst; plasma membrane; cytosol Molecular Function:protein binding; Ral GTPase binding; protein N-terminus binding; protein kinase binding Biological Process: Golgi to plasma membrane transport; protein transport; cellular protein metabolic process; exocytosis; organelle organization and biogenesis
NCBI Summary:	The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
UniProt Code:	Q96KP1
NCBI GenInfo Identifier:	15982242
NCBI Gene ID:	55770
NCBI Accession:	CAC92092.1
UniProt Secondary Accession:	Q96KP1,Q5JPC8, Q96AN6, Q9NUZ8, Q9UJM7, B2RBE6,
UniProt Related Accession:	Q96KP1
Molecular Weight:	104,066 Da
NCBI Full Name:	Sec5 protein
NCBI Synonym Full Names:	exocyst complex component 2
NCBI Official Symbol:	EXOC2
NCBI Official Synonym Symbols:	SEC5; Sec5p; SEC5L1
NCBI Protein Information:	exocyst complex component 2
UniProt Protein Name:	Exocyst complex component 2
UniProt Synonym Protein Names:	Exocyst complex component Sec5
Protein Family:	Exocyst complex component
UniProt Gene Name:	EXOC2
UniProt Entry Name:	EXOC2_HUMAN

	Western blot analysis of extracts of various cell lines, using EXOC2 antibody (CAB8235) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using EXOC2 antibody (CAB8235) at dilution of 1:100. Blue: DAPI for nuclear staining.