Cell Biology Antibodies 6

Anti-ETFB Antibody (CAB16043)

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SKU:
CAB16043
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ETFB Antibody
Antibody SKU:CAB16043
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-230 of human ETFB (NP_001976.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-549, 293T, BxPC-3, Mouse brain, Mouse heart, Mouse kidney, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 80-230 of human ETFB (NP_001976.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AMGA DRGI HVEV PPAE AERL GPLQ VARV LAKL AEKE KVDL VLLG KQAI DDDC NQTG QMTA GFLD WPQG TFAS QVTL EGDK LKVE REID GGLE TLRL KLPA VVTA DLRL NEPR YATL PNIM KAKK KKIE VIKP GDLG VDLT SKLS VISV EDP
Gene ID:2109
Uniprot:P38117
Cellular Location:Mitochondrion matrix
Calculated MW:27kDa/37kDa
Observed MW:30kDa
Synonyms:ETFB, FP585, MADD
Background:This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:ETFB: The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B). GA2B is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Belongs to the ETF beta-subunit/FixA family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix

Molecular Function:electron carrier activity

Biological Process: cellular metabolic process

Disease: Multiple Acyl-coa Dehydrogenase Deficiency

NCBI Summary:This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P38117
NCBI GenInfo Identifier:62420877
NCBI Gene ID:2109
NCBI Accession:NP_001014763.1
UniProt Secondary Accession:P38117,Q6IBH7, Q71RF6, Q9Y3S7, B3KNY2,
UniProt Related Accession:P38117
Molecular Weight:37,566 Da
NCBI Full Name:electron transfer flavoprotein subunit beta isoform 2
NCBI Synonym Full Names:electron-transfer-flavoprotein, beta polypeptide
NCBI Official Symbol:ETFB  
NCBI Official Synonym Symbols:MADD; FP585  
NCBI Protein Information:electron transfer flavoprotein subunit beta; beta-ETF; electron transfer flavoprotein beta subunit; electron transfer flavoprotein beta-subunit; electron transfer flavoprotein, beta polypeptide; electron-transferring-flavoprotein, beta polypeptide
UniProt Protein Name:Electron transfer flavoprotein subunit beta
Protein Family:Electron transfer flavoprotein
UniProt Gene Name:ETFB  
UniProt Entry Name:ETFB_HUMAN
Anti-ETFB Antibody (CAB16043)Western blot analysis of extracts of various cell lines, using ETFB antibody (CAB16043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-ETFB Antibody (CAB16043)Immunofluorescence analysis of C6 cells using ETFB Polyclonal Antibody (CAB16043) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-ETFB Antibody (CAB16043)Immunofluorescence analysis of L929 cells using ETFB Polyclonal Antibody (CAB16043) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-ETFB Antibody (CAB16043)Immunofluorescence analysis of U-2 OS cells using ETFB Polyclonal Antibody (CAB16043) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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