Name: Anti-ESYT1 Antibody (CAB15410)
Brand: Cell Biology Antibodies 6
SKU: CAB15410
Availability: OutOfStock

Antibody Name:	Anti-ESYT1 Antibody
Antibody SKU:	CAB15410
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-220 of human ESYT1 (NP_056107.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, 293T, LO2, Jurkat, HeLa, Mouse brain, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-220 of human ESYT1 (NP_056107.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GWRR VRDE KERS LRAA RQLL DDEE QLTA KTLY MSHR ELPA WVSF PDVE KAEW LNKI VAQV WPFL GQYM EKLL AETV APAV RGSN PHLQ TFTF TRVE LGEK PLRI IGVK VHPG QRKE QILL DLNI SYVG DVQ
Gene ID:	23344
Uniprot:	Q9BSJ8
Cellular Location:	Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein, Peripheral membrane protein
Calculated MW:	122kDa/124kDa
Observed MW:	123kDa

Synonyms:	ESYT1, FAM62A, MBC2
Background:

UniProt Protein Function:	ESYT1: May play a role as calcium-regulated intrinsic membrane protein. Belongs to the extended synaptotagmin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 12q13.2 Cellular Component: integral to endoplasmic reticulum membrane; membrane Molecular Function:protein binding
UniProt Code:	Q9BSJ8
NCBI GenInfo Identifier:	74733019
NCBI Gene ID:	23344
NCBI Accession:	Q9BSJ8.1
UniProt Secondary Accession:	Q9BSJ8,O94848, Q6PJN4, Q9H6J1, Q9H6W2, Q9Y416, A0FGR7 A8K2S2,
UniProt Related Accession:	Q9BSJ8
Molecular Weight:	124,003 Da
NCBI Full Name:	Extended synaptotagmin-1
NCBI Synonym Full Names:	extended synaptotagmin 1
NCBI Official Symbol:	ESYT1
NCBI Official Synonym Symbols:	MBC2; FAM62A
NCBI Protein Information:	extended synaptotagmin-1
UniProt Protein Name:	Extended synaptotagmin-1
UniProt Synonym Protein Names:	Membrane-bound C2 domain-containing protein
Protein Family:	Extended synaptotagmin
UniProt Gene Name:	ESYT1
UniProt Entry Name:	ESYT1_HUMAN

	Western blot analysis of extracts of various cell lines, using ESYT1 antibody (CAB15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of U-2 OS cells using ESYT1 antibody (CAB15410) at dilution of 1:100. Blue: DAPI for nuclear staining.