Epigenetics & Nuclear Signaling Antibodies 3

Anti-ESRP1 Antibody (CAB14626)

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SKU:
CAB14626
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-ESRP1 Antibody
Antibody SKU:CAB14626
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ESRP1 (NP_060167.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, MCF7, HT-29, 293T, Mouse thymus, Mouse pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ESRP1 (NP_060167.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTAS PDYL VVLF GITA GATG AKLG SDEK ELIL LFWK VVDL ANKK VGQL HEVL VRPD QLEL TEDC KEET KIDV ESLS SASQ LDQA LRQF NQSV SNEL NIGV GTSF CLCT DGQL HVRQ ILHP EASK KNVL LPEC FYSF FDLR KEFK KCCP GSPD IDKL DVAT MTEY LNFE KSSS VSRY GASQ VEDM GNII LAMI SEPY NHRF SDPE RVNY KFES GTCS KMEL
Gene ID:54845
Uniprot:Q6NXG1
Cellular Location:Nucleus
Calculated MW:67kDa/73kDa/75kDa
Observed MW:75kDa
Synonyms:ESRP1, RBM35A, RMB35A
Background:
UniProt Protein Function:RBM35A: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Belongs to the ESRP family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA processing

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: nucleus

Molecular Function:mRNA binding; nucleotide binding

Biological Process: regulation of RNA splicing; RNA splicing; mRNA processing

NCBI Summary:ESPR1 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009]
UniProt Code:Q6NXG1
NCBI GenInfo Identifier:170763525
NCBI Gene ID:54845
NCBI Accession:NP_001030087.2
UniProt Secondary Accession:Q6NXG1,Q2M2B0, Q499G3, Q6PJ86, Q9NXL8, A6NHA8, A8MPX1 E9PB47,
UniProt Related Accession:Q6NXG1
Molecular Weight:75585
NCBI Full Name:epithelial splicing regulatory protein 1 isoform 2
NCBI Synonym Full Names:epithelial splicing regulatory protein 1
NCBI Official Symbol:ESRP1  
NCBI Official Synonym Symbols:RBM35A; RMB35A  
NCBI Protein Information:epithelial splicing regulatory protein 1
UniProt Protein Name:Epithelial splicing regulatory protein 1
UniProt Synonym Protein Names:RNA-binding motif protein 35A; RNA-binding protein 35A
Protein Family:Epithelial splicing regulatory protein
UniProt Gene Name:ESRP1  
UniProt Entry Name:ESRP1_HUMAN
Anti-ESRP1 Antibody (CAB14626)Western blot analysis of extracts of various cell lines, using ESRP1 antibody (CAB14626) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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