Description
| Antibody Name: | Anti-ERp19 Antibody (CAB9386) | 
| Antibody SKU: | CAB9386 | 
| Antibody Size: | 50µL, 100µL | 
| Application: | Western blotting, Immunohistochemistry | 
| Reactivity: | Human, Mouse, Rat | 
| Host Species: | Rabbit | 
| Immunogen: | A synthesized peptide derived from human ERp19. | 
| Application: | Western blotting, Immunohistochemistry | 
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200  | 
| Reactivity: | Human, Mouse, Rat | 
| Positive Samples: | HT-29, 293T, SH-SY5Y, Mouse lung, Mouse heart, Mouse placenta, Rat lung, Rat kidney, Rat heart | 
| Immunogen: | A synthesized peptide derived from human ERp19. | 
| Purification Method: | Affinity purification | 
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.  | 
| Isotype: | IgG | 
| Sequence: | Email for sequence | 
| Cellular Location: | Endoplasmic reticulum lumen | 
| Calculated MW: | 17kDa | 
| Observed MW: | 19KDa | 
| Synonyms: | TXNDC12, AG1, AGR1, ERP16, ERP18, ERP19, PDIA16, TLP19, hAG-1, hTLP19, thioredoxin domain containing 12 | 
| Background: | This gene encodes a member of the thioredoxin superfamily. Members of this family are characterized by a conserved active motif called the thioredoxin fold that catalyzes disulfide bond formation and isomerization. This protein localizes to the endoplasmic reticulum and has a single atypical active motif. The encoded protein is mainly involved in catalyzing native disulfide bond formation and displays activity similar to protein-disulfide isomerases. This protein may play a role in defense against endoplasmic reticulum stress. Alternate splicing results in both coding and non-coding variants. | 
![]()  | Immunohistochemistry of paraffin-embedded mouse stomach using ERp19 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | 
![]()  | Immunohistochemistry of paraffin-embedded rat brain using ERp19 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | 
![]()  | Immunohistochemistry of paraffin-embedded mouse kidney using ERp19 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | 
![]()  | Immunohistochemistry of paraffin-embedded human tonsil using ERp19 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | 
![]()  | Immunohistochemistry of paraffin-embedded human lung cancer using ERp19 Rabbit mAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | 
![]()  | Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. | 
![]()  | Western blot analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s. | 
            





