Cell Biology Antibodies 1

ErbB3/HER3 Rabbit Polyclonal Antibody (CAB0950)

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SKU:
CAB0950
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Reactivity:
Human, Mouse
Synonyms:
HER3, FERLK, LCCS2, VSCN1, ErbB-3, c-erbB3, erbB3-S, MDA-BF-1, c-erbB-3, p180-ErbB3, p45-sErbB3, p85-sErbB3, R3
Research Use:
Cell Biology
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Description

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ErbB3/HER3 Antibody (CAB0950)

The ErbB3/HER3 Antibody (CAB0950) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications.ERBB3 is known to play a critical role in cell growth, survival, and differentiation, particularly in the development of various cancers. By binding to the ERBB3 protein, this antibody allows for the detection and analysis of ERBB3 expression in a variety of cell types, providing researchers with crucial insights into the role of ERBB3 in cancer progression and treatment.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:ErbB3/HER3 Antibody
SKU:CAB0950
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:DYAA MGAC PASE QGYE EMRA FQGP GHQA PHVH YARL KTLR SLEA TDSA FDNP DYWH SRLF PKAN AQRT
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:HER3, FERLK, LCCS2, VSCN1, ErbB-3, c-erbB3, erbB3-S, MDA-BF-1, c-erbB-3, p180-ErbB3, p45-sErbB3, p85-sErbB3, R3
Positive Sample:293T, MCF7, A-431, Mouse liver
Cellular Localization:Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:148kDa
Observed MW:185kDa

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

Purification MethodAffinity purification
Gene ID2065
RRIDAB_2757474
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] ErbB3/HER3 Rabbit pAb (CAB0950) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Product ImageWestern blot analysis of lysates from wild type (WT) and ErbB3/HER3 knockout (KO) 293T cells, using [KO Validated] ErbB3/HER3 Rabbit pAb (CAB0950) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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