Signal Transduction Antibodies 2

Anti-EPX Antibody (CAB16450)

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SKU:
CAB16450
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-EPX Antibody
Antibody SKU:CAB16450
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EPX (NP_000493.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:SW620, human serum
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EPX (NP_000493.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TELR RLNP RWNG DKLY NEAR KIMG AMVQ IITY RDFL PLVL GKAR ARRT LGHY RGYC SNVD PRVA NVFT LAFR FGHT MLQP FMFR LDSQ YRAS APNS HVPL S
Gene ID:8288
Uniprot:P11678
Cellular Location:Cytoplasmic granule
Calculated MW:81kDa
Observed MW:81kDa
Synonyms:EPX, EPO, EPP, EPX-PEN, EPXD
Background:This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded preproprotein is proteolytically processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a gene cluster with other peroxidase genes on chromosome 17. Mutations in this gene result in eosinophil peroxidase deficiency.
UniProt Protein Function:EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.11.1.7

Chromosomal Location of Human Ortholog: 17q23.1

Molecular Function:peroxidase activity; metal ion binding; heme binding

Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; hydrogen peroxide catabolic process; negative regulation of interleukin-5 production; response to oxidative stress; defense response to nematode

Disease: Eosinophil Peroxidase Deficiency

UniProt Code:P11678
NCBI GenInfo Identifier:4503595
NCBI Gene ID:8288
NCBI Accession:
UniProt Secondary Accession:P11678,Q4TVP3,
Molecular Weight:
NCBI Full Name:eosinophil peroxidase preproprotein
NCBI Synonym Full Names:eosinophil peroxidase
NCBI Official Symbol:EPX  
NCBI Official Synonym Symbols:EPO; EPP; EPXD; EPX-PEN  
NCBI Protein Information:eosinophil peroxidase
UniProt Protein Name:Eosinophil peroxidase
Protein Family:Eosinophil peroxidase
UniProt Gene Name:EPX  
UniProt Entry Name:PERE_HUMAN
Anti-EPX Antibody (CAB16450)Western blot analysis of extracts of various cell lines, using EPX antibody (CAB16450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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