Cell Biology Antibodies 12

Anti-EPS15 Antibody (CAB9814)

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SKU:
CAB9814
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EPS15 Antibody
Antibody SKU:CAB9814
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 660-840 of human EPS15 (NP_001972.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, Jurkat, 293T, NIH/3T3, Mouse liver, Mouse spleen, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 660-840 of human EPS15 (NP_001972.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RQST DPFA TSST DPFS AANN SSIT SVET LKHN DPFA PGGT VVAA SDSA TDPF ASVF GNES FGGG FADF STLS KVNN EDPF RSAT SSSV SNVV ITKN VFEE TSVK SEDE PPAL PPKI GTPT RPCP LPPG KRSI NKLD SPDP FKLN DPFQ PFPG NDSP KEKD PEIF CDPF TSAT TTTN KEAD P
Gene ID:2060
Uniprot:P42566
Cellular Location:Cell membrane, Cytoplasm, Cytoplasmic side, Early endosome membrane, Membrane, Peripheral membrane protein, clathrin-coated pit
Calculated MW:64kDa/98kDa
Observed MW:150kDa
Synonyms:EPS15, AF-1P, AF1P, MLLT5
Background:This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
NCBI Summary:This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009]
UniProt Code:P42566
NCBI GenInfo Identifier:67476728
NCBI Gene ID:2060
NCBI Accession:P42566.2
UniProt Secondary Accession:P42566,Q5SRH4, B2R8J7, D3DPJ2,
UniProt Related Accession:P42566
Molecular Weight:99kDa
NCBI Full Name:Epidermal growth factor receptor substrate 15
NCBI Synonym Full Names:epidermal growth factor receptor pathway substrate 15
NCBI Official Symbol:EPS15  
NCBI Official Synonym Symbols:AF1P; AF-1P; MLLT5  
NCBI Protein Information:epidermal growth factor receptor substrate 15
UniProt Protein Name:Epidermal growth factor receptor substrate 15
UniProt Synonym Protein Names:Protein AF-1p
Protein Family:Epidermal growth factor receptor substrate
UniProt Gene Name:EPS15  
Anti-EPS15 Polyclonal Antibody (CAB9814)Western blot analysis of extracts of various cell lines, using EPS15 antibody (CAB9814) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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