Cell Biology Antibodies 7
Anti-Epn2 Antibody (CAB16623)
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- SKU:
- CAB16623
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Epn2 Antibody |
| Antibody SKU: | CAB16623 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse Epn2 (XP_006532226.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HepG2, Mouse lung, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of mouse Epn2 (XP_006532226.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IFAI QTLK DFQY IDRD GKDQ GINV REKS KQLV ALLK DEER LKVE RVQA LKTK ERMA QVAT GVGS NQIT FGRG SSQP NLST SYSE QEYG KAGG SPAS YHGS P |
| Gene ID: | 13855 |
| Uniprot: | O95208 |
| Cellular Location: | |
| Calculated MW: | 36kDa/57kDa/62kDa/68kDa |
| Observed MW: | 68kDa |
| Synonyms: | 9530051D10Rik, AA536924, Ibp2, Epn2 |
| Background: |
| UniProt Protein Function: | epsin 2: Plays a role in the formation of clathrin-coated invaginations and endocytosis. Belongs to the epsin family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm Molecular Function:lipid binding; protein binding Biological Process: embryonic organ development; endocytosis; in utero embryonic development; Notch signaling pathway; regulation of endocytosis |
| NCBI Summary: | This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95208 |
| NCBI GenInfo Identifier: | 218512093 |
| NCBI Gene ID: | 22905 |
| NCBI Accession: | O95208.3 |
| UniProt Secondary Accession: | O95208,O95207, Q52LD0, Q9H7Z2, Q9UPT7, A8MTV8, B3KRX8 E9PBC2, |
| UniProt Related Accession: | O95208 |
| Molecular Weight: | 36,729 Da |
| NCBI Full Name: | Epsin-2 |
| NCBI Synonym Full Names: | epsin 2 |
| NCBI Official Symbol: | EPN2 |
| NCBI Official Synonym Symbols: | EHB21 |
| NCBI Protein Information: | epsin-2 |
| UniProt Protein Name: | Epsin-2 |
| UniProt Synonym Protein Names: | EPS-15-interacting protein 2 |
| UniProt Gene Name: | EPN2 |
| UniProt Entry Name: | EPN2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Epn2 antibody (CAB16623) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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