Cell Biology Antibodies 6

Anti-EPN2 Antibody (CAB16106)

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SKU:
CAB16106
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-EPN2 Antibody
Antibody SKU:CAB16106
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-300 of human EPN2 (NP_683723.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human
Positive Samples:U-87MG, LO2, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 120-300 of human EPN2 (NP_683723.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GINV REKS KQLV ALLK DEER LKAE RAQA LKTK ERMA QVAT GMGS NQIT FGRG SSQP NLST SHSE QEYG KAGG SPAS YHGS TSPR VSSE LEQA RPQT SGEE ELQL QLAL AMSR EVAE QEER LRRG DDLR LQMA LEES RRDT VKIP KKKE HGSL PQQT TLLD LMDA LPSS GPAA QKAE PWGP S
Gene ID:22905
Uniprot:O95208
Cellular Location:Cytoplasm, Cytoplasmic vesicle, clathrin-coated vesicle
Calculated MW:36kDa/57kDa/62kDa/68kDa
Observed MW:70kDa
Synonyms:EPN2, EHB21, epsin-2
Background:This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:epsin 2: Plays a role in the formation of clathrin-coated invaginations and endocytosis. Belongs to the epsin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm

Molecular Function:lipid binding; protein binding

Biological Process: embryonic organ development; endocytosis; in utero embryonic development; Notch signaling pathway; regulation of endocytosis

NCBI Summary:This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:O95208
NCBI GenInfo Identifier:218512093
NCBI Gene ID:22905
NCBI Accession:O95208.3
UniProt Secondary Accession:O95208,O95207, Q52LD0, Q9H7Z2, Q9UPT7, A8MTV8, B3KRX8 E9PBC2,
UniProt Related Accession:O95208
Molecular Weight:36,729 Da
NCBI Full Name:Epsin-2
NCBI Synonym Full Names:epsin 2
NCBI Official Symbol:EPN2  
NCBI Official Synonym Symbols:EHB21  
NCBI Protein Information:epsin-2
UniProt Protein Name:Epsin-2
UniProt Synonym Protein Names:EPS-15-interacting protein 2
UniProt Gene Name:EPN2  
UniProt Entry Name:EPN2_HUMAN
Anti-EPN2 Antibody (CAB16106)Western blot analysis of extracts of various cell lines, using EPN2 antibody (CAB16106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
Anti-EPN2 Antibody (CAB16106)Immunohistochemistry of paraffin-embedded Human colon carcinoma using EPN2 Rabbit pAb (CAB16106) at dilution of 1:100 (40x lens).
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