Antibody Name:	Anti-ENPP3 Antibody
Antibody SKU:	CAB14763
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 646-875 of human ENPP3 (NP_005012.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	DU145, HeLa, HT-29, Mouse lung, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 646-875 of human ENPP3 (NP_005012.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YTVP QLGD TSPL PPTV PDCL RADV RVPP SESQ KCSF YLAD KNIT HGFL YPPA SNRT SDSQ YDAL ITSN LVPM YEEF RKMW DYFH SVLL IKHA TERN GVNV VSGP IFDY NYDG HFDA PDEI TKHL ANTD VPIP THYF VVLT SCKN KSHT PENC PGWL DVLP FIIP HRPT NVES CPEG KPEA LWVE ERFT AHIA RVRD VELL TGLD FYQD KVQP VSEI LQLK TYLP TFET TI
Gene ID:	5169
Uniprot:	O14638
Cellular Location:	Membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	100kDa
Observed MW:	110kDa

Synonyms:

ENPP3, B10, CD203c, NPP3, PD-IBETA, PDNP3

Background:

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.

UniProt Protein Function:	ENPP3: Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Nucleotide Metabolism - purine; EC 3.1.4.1; Cofactor and Vitamin Metabolism - riboflavin; Phosphodiesterase; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Motility/polarity/chemotaxis; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 3.6.1.9 Chromosomal Location of Human Ortholog: 6q22 Cellular Component: integral to plasma membrane; perinuclear region of cytoplasm Molecular Function:phosphodiesterase I activity; nucleotide diphosphatase activity; nucleic acid binding; metal ion binding; nucleoside-triphosphate diphosphatase activity; scavenger receptor activity; polysaccharide binding Biological Process: receptor-mediated endocytosis; nucleoside triphosphate catabolic process; immune response; phosphate metabolic process; inorganic diphosphate transport
NCBI Summary:	The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. [provided by RefSeq, Jul 2008]
UniProt Code:	O14638
NCBI GenInfo Identifier:	206729860
NCBI Gene ID:	5169
NCBI Accession:	O14638.2
UniProt Secondary Accession:	O14638,Q5JTL3,
UniProt Related Accession:	O14638
Molecular Weight:	100,124 Da
NCBI Full Name:	Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
NCBI Synonym Full Names:	ectonucleotide pyrophosphatase/phosphodiesterase 3
NCBI Official Symbol:	ENPP3
NCBI Official Synonym Symbols:	B10; NPP3; PDNP3; CD203c; PD-IBETA
NCBI Protein Information:	ectonucleotide pyrophosphatase/phosphodiesterase family member 3; gp130RB13-6; phosphodiesterase-I beta; phosphodiesterase I/nucleotide pyrophosphatase 3; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3)
UniProt Protein Name:	Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
UniProt Synonym Protein Names:	Phosphodiesterase I beta; PD-Ibeta; Phosphodiesterase I/nucleotide pyrophosphatase 3
UniProt Gene Name:	ENPP3
UniProt Entry Name:	ENPP3_HUMAN

	Western blot analysis of extracts of various cell lines, using ENPP3 Rabbit pAb (CAB14763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human gastric cancer using ENPP3 antibody (CAB14763) at dilution of 1:150 (40x lens).