Anti-eNOS Antibody (CAB20985)
- SKU:
- CAB20985
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-eNOS Antibody (CAB20985) |
| Antibody SKU: | CAB20985 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1110 of human eNOS (NP_000594.2). |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | PC-3, HUVEC, Mouse liver, Mouse placenta, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1035-1110 of human eNOS (NP_000594.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KGLQ PTPM TLVF GCRC SQLD HLYR DEVQ NAQQ RGVF GRVL TAFS REPD NPKT YVQD ILRT ELAA EVHR VLCL ERGH |
| Cellular Location: | Cell membrane, Cytoplasm, Golgi apparatus, Membrane, caveola, cytoskeleton |
| Calculated MW: | 67kDa/68kDa/133kDa |
| Observed MW: | 148KDa |
| Synonyms: | NOS3, ECNOS, eNOS |
| Background: | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
 | Western blot analysis of extracts of various cell lines, using eNOS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |
Related Products
eNOS Monoclonal Antibody (CAB22344)
eNOS Monoclonal Antibody (CAB22344)The product page for this antibody is being prepared. If you have a question please don't hesitate to get in touch!