Description
Antibody Name: | Anti-ELOVL6 Antibody (CAB21094) |
Antibody SKU: | CAB21094 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ELOVL6 (NP_076995.1). |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | U-251MG, HepG2, SK-BR-3, Mouse liver, Rat liver |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ELOVL6 (NP_076995.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | LMNK RAKF ELRK PLVL WSLT LAVF SIFG ALRT GAYM VYIL MTKG LKQS VCDQ GFYN GPVS KFWA YAFV LSKA PELG DTIF IILR KQKL IFLH WYHH ITVL L |
Cellular Location: | |
Calculated MW: | 31kDa |
Observed MW: | 30KDa/45KDa |
Synonyms: | FAE, LCE, FACE, hELO2 |
Background: | Fatty acid elongases (EC 6.2.1.3), such as ELOVL6, use malonyl-CoA as a 2-carbon donor in the first and rate-limiting step of fatty acid elongation (Moon et al., 2001 PubMed 11567032). |
Western blot analysis of extracts of various cell lines, using ELOVL6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |