Antibody Name:	Anti-ELF5 Antibody
Antibody SKU:	CAB7181
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ELF5 (NP_001413.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat lung, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ELF5 (NP_001413.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLDS VTHS TFLP NASF CDPL MSWT DLFS NEEY YPAF EHQT ACDS YWTS VHPE YWTK RHVW EWLQ FCCD QYKL DTNC ISFC NFNI SGLQ LCSM TQEE FVEA AGLC GEYL YFIL QNIR TQGY SFFN DAEE SKAT IKDY ADSN CLKT SGIK SQDC HSHS RTSL QSSH LWEF VR
Gene ID:	2001
Uniprot:	Q9UKW6
Cellular Location:	Nucleus
Calculated MW:	16kDa/18kDa/30kDa/31kDa
Observed MW:	31KDa

Synonyms:

ELF5, ESE2

Background:

The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	ELF5: Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Belongs to the ETS family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 11p13-p12 Cellular Component: nucleus Biological Process: cell differentiation; cell proliferation; regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
UniProt Code:	Q9UKW6
NCBI GenInfo Identifier:	68565549
NCBI Gene ID:	2001
NCBI Accession:	Q9UKW6.2
UniProt Secondary Accession:	Q9UKW6,O95175, Q8N2K9, Q96QY3, Q9UKW5, A6XAE6, A8K452
UniProt Related Accession:	Q9UKW6
Molecular Weight:	18,866 Da
NCBI Full Name:	ETS-related transcription factor Elf-5
NCBI Synonym Full Names:	E74 like ETS transcription factor 5
NCBI Official Symbol:	ELF5
NCBI Official Synonym Symbols:	ESE2
NCBI Protein Information:	ETS-related transcription factor Elf-5
UniProt Protein Name:	ETS-related transcription factor Elf-5
UniProt Synonym Protein Names:	E74-like factor 5; Epithelium-restricted ESE-1-related Ets factor; Epithelium-specific Ets transcription factor 2; ESE-2
Protein Family:	ETS-related transcription factor
UniProt Gene Name:	ELF5
UniProt Entry Name:	ELF5_HUMAN

	Western blot analysis of extracts of various cell lines, using ELF5 antibody (CAB7181) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat liver using ELF5 Rabbit pAb (CAB7181) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using ELF5 Rabbit pAb (CAB7181) at dilution of 1:50 (40x lens).
	Immunofluorescence analysis of A549 cells using ELF5 antibody (CAB7181). Blue: DAPI for nuclear staining.