Epigenetics & Nuclear Signaling Antibodies 3

eIF5A Rabbit Polyclonal Antibody (CAB2016)

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SKU:
CAB2016
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse
Synonyms:
FABAS, EIF-5A, EIF5A1, eIF-4D, eIF5AI, eIF5A
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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eIF5A Antibody (CAB2016)

The eIF5A Antibody (CAB2016) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human eIF5A and is validated for use in Western blot applications. By binding to eIF5A, this antibody allows for the detection and analysis of the protein in various cell types, making it an ideal choice for studies in molecular biology and cancer research.eIF5A is known to play a crucial role in the translation of specific proteins that are essential for cell growth and differentiation.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:eIF5A Antibody
SKU:CAB2016
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MADD LDFE TGDA GASA TFPM QCSA LRKN GFVV LKGR PCKI VEMS TSKT GKHG HAKV HLVG IDIF TGKK YEDI CPST HNMD VPNI KRND FQLI GIQD GYLS LLQD SGEV REDL RLPE GDLG KEIE QKYD CGEE ILIT VLSA MTEE AAVA IKAM AK
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:2000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:FABAS, EIF-5A, EIF5A1, eIF-4D, eIF5AI, eIF5A
Positive Sample:HeLa, 293T, Raji, 22Rv1, HepG2, K-562, U-251MG, Mouse liver, Mouse testis
Cellular Localization:Cytoplasm, Cytoplasmic Side, Endoplasmic Reticulum Membrane, Nucleus, Peripheral Membrane Protein, Nuclear Pore Complex.
Calculated MW:17kDa
Observed MW:17kDa

Enables U6 snRNA binding activity and protein N-terminus binding activity. Involved in several processes, including cellular response to virus; positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator; and tumor necrosis factor-mediated signaling pathway. Located in annulate lamellae; cytoplasm; and nucleus. Part of nuclear pore.

Purification MethodAffinity purification
Gene ID1984
RRIDAB_2764040
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using eIF5A Rabbit pAb (CAB2016) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer using eIF5A Rabbit pAb (CAB2016) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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