Name: Anti-EIF5A Antibody (CAB2016)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB2016
Availability: OutOfStock

Antibody Name:	Anti-EIF5A Antibody
Antibody SKU:	CAB2016
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human EIF5A (NP_001137234.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, 293T, Raji, 22Rv1, HepG2, K-562, U-251MG, Mouse liver, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human EIF5A (NP_001137234.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADD LDFE TGDA GASA TFPM QCSA LRKN GFVV LKGR PCKI VEMS TSKT GKHG HAKV HLVG IDIF TGKK YEDI CPST HNMD VPNI KRND FQLI GIQD GYLS LLQD SGEV REDL RLPE GDLG KEIE QKYD CGEE ILIT VLSA MTEE AAVA IKAM AK
Gene ID:	1984
Uniprot:	P63241
Cellular Location:	Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Nucleus, Peripheral membrane protein, nuclear pore complex
Calculated MW:	16kDa/20kDa
Observed MW:	17kDa

Synonyms:	EIF5A, EIF-5A, EIF5A1, eIF5AI
Background:

UniProt Protein Function:	EIF5A: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. Belongs to the eIF-5A family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 17p13-p12 Cellular Component: endoplasmic reticulum membrane; membrane; cytoplasm; nuclear pore; cytosol; nucleus; annulate lamellae Molecular Function:protein binding; RNA binding; ribosome binding; protein N-terminus binding; translation elongation factor activity; U6 snRNA binding Biological Process: mRNA export from nucleus; translational frameshifting; cellular protein metabolic process; apoptosis; positive regulation of cell proliferation; nucleocytoplasmic transport; positive regulation of translational elongation; hypusine biosynthetic process from peptidyl-lysine; protein export from nucleus; post-translational protein modification; positive regulation of translational termination
UniProt Code:	P63241
NCBI GenInfo Identifier:	54037409
NCBI Gene ID:	1984
NCBI Accession:	P63241.2
UniProt Related Accession:	P63241
Molecular Weight:	~ 18kDa
NCBI Full Name:	Eukaryotic translation initiation factor 5A-1
NCBI Synonym Full Names:	eukaryotic translation initiation factor 5A
NCBI Official Symbol:	EIF5A
NCBI Official Synonym Symbols:	EIF-5A; EIF5A1; eIF-4D; eIF5AI
NCBI Protein Information:	eukaryotic translation initiation factor 5A-1
UniProt Protein Name:	Eukaryotic translation initiation factor 5A-1
UniProt Synonym Protein Names:	Eukaryotic initiation factor 5A isoform 1; eIF-5A; Rev-binding factor; eIF-4D
Protein Family:	Translation initiation factor
UniProt Gene Name:	EIF5A
UniProt Entry Name:	IF5A1_HUMAN

	Western blot analysis of extracts of various cell lines, using EIF5A antibody (CAB2016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human breast cancer using EIF5A Rabbit pAb (CAB2016) at dilution of 1:25 (40x lens).
	Immunofluorescence analysis of U2OS cells using EIF5A antibody (CAB2016). Blue: DAPI for nuclear staining.