Metabolism Antibodies 2
Anti-EIF5 Antibody (CAB6583)
- SKU:
- CAB6583
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-EIF5 Antibody |
| Antibody SKU: | CAB6583 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human EIF5 (NP_892116.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, SW480, HeLa, U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human EIF5 (NP_892116.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSVN VNRS VSDQ FYRY KMPR LIAK VEGK GNGI KTVI VNMV DVAK ALNR PPTY PTKY FGCE LGAQ TQFD VKND RYIV NGSH EANK LQDM LDGF IKKF VLCP ECEN PETD LHVN PKKQ TIGN SCKA CGYR GMLD THHK LCTF ILKN PPEN SDSG TGKK EKEK KNRK GKDK ENGS VSSS ETPP PPPP PNEI NPPP HTME EEED DDWG EDTT EEAQ RRRM DEIS DHAK VLTL SDDL ERT |
| Gene ID: | 1983 |
| Uniprot: | P55010 |
| Cellular Location: | |
| Calculated MW: | 49kDa |
| Observed MW: | 55kDa |
| Synonyms: | EIF5, EIF-5, EIF-5A |
| Background: |
| UniProt Protein Function: | EIF5: eukaryotic translation initiation factor 5. Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex. |
| UniProt Protein Details: | Protein type:Translation initiation; Translation Chromosomal Location of Human Ortholog: 14q32.32 Cellular Component: cytoplasm; cytosol; nucleus; plasma membrane Molecular Function:GTPase activity; protein binding; translation factor activity, nucleic acid binding Biological Process: regulation of translational initiation |
| NCBI Summary: | Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010] |
| UniProt Code: | P55010 |
| NCBI GenInfo Identifier: | 27735202 |
| NCBI Gene ID: | 1983 |
| NCBI Accession: | P55010.2 |
| UniProt Secondary Accession: | P55010,Q53XB3, Q9H5N2, Q9UG48, |
| UniProt Related Accession: | P55010 |
| Molecular Weight: | 49,223 Da |
| NCBI Full Name: | Eukaryotic translation initiation factor 5 |
| NCBI Synonym Full Names: | eukaryotic translation initiation factor 5 |
| NCBI Official Symbol: | EIF5 |
| NCBI Official Synonym Symbols: | EIF-5; EIF-5A |
| NCBI Protein Information: | eukaryotic translation initiation factor 5 |
| UniProt Protein Name: | Eukaryotic translation initiation factor 5 |
| UniProt Gene Name: | EIF5 |
| UniProt Entry Name: | IF5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EIF5 antibody (CAB6583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using EIF5 antibody (CAB6583). Blue: DAPI for nuclear staining. |
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