Immunology Antibodies 1

Anti-EIF3A Antibody (CAB0573)

(No reviews yet) Write a Review
SKU:
CAB0573
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-EIF3A Antibody
Antibody SKU:CAB0573
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF3A (NP_003741.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:HepG2, Jurkat, Mouse heart, Mouse ovary
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF3A (NP_003741.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPAY FQRP ENAL KRAN EFLE VGKK QPAL DVLY DVMK SKKH RTWQ KIHE PIML KYLE LCVD LRKS HLAK EGLY QYKN ICQQ VNIK SLED VVRA YLKM AEEK TEAA KEES QQMV LDIE DLDN IQTP ESVL LSAV SGED TQDR TDRL LLTP WVKF LWES YRQC LDLL RNNS RVER LYHD IAQQ AFKF CLQY TRKA EFRK LCDN LRMH LSQI QRHH NQST AINL NNPE SQSM HLET RLVQ LDSA ISME LWQE AF
Gene ID:8661
Uniprot:Q14152
Cellular Location:Cytoplasm
Calculated MW:162kDa/166kDa
Observed MW:180kDa
Synonyms:EIF3A, EIF3, EIF3S10, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185
Background:
UniProt Protein Function:eIF3-theta: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Interacts with EIF4G1. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2. Belongs to the eIF-3 subunit A family.
UniProt Protein Details:

Protein type:Translation; RNA-binding; Translation initiation

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane; nucleolus; nucleus

Molecular Function:protein binding; translation initiation factor activity

Biological Process: formation of translation initiation complex; translational initiation

UniProt Code:Q14152
NCBI GenInfo Identifier:6685537
NCBI Gene ID:8661
NCBI Accession:Q14152.1
UniProt Secondary Accession:Q14152,O00653, Q15778, B1AMV5, B4DYS1, F5H335,
UniProt Related Accession:Q14152
Molecular Weight:162,636 Da
NCBI Full Name:Eukaryotic translation initiation factor 3 subunit A
NCBI Synonym Full Names:eukaryotic translation initiation factor 3 subunit A
NCBI Official Symbol:EIF3A  
NCBI Official Synonym Symbols:EIF3; P167; p180; p185; TIF32; EIF3S10; eIF3-p170; eIF3-theta  
NCBI Protein Information:eukaryotic translation initiation factor 3 subunit A
UniProt Protein Name:Eukaryotic translation initiation factor 3 subunit A
UniProt Synonym Protein Names:Eukaryotic translation initiation factor 3 subunit 10
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF3A  
UniProt Entry Name:EIF3A_HUMAN
Anti-EIF3A Antibody (CAB0573)Western blot analysis of extracts of various cell lines, using EIF3A antibody (CAB0573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-EIF3A Antibody (CAB0573)Immunohistochemistry of paraffin-embedded human stomach using EIF3A antibody (CAB0573) at dilution of 1:100 (40x lens).
Anti-EIF3A Antibody (CAB0573)Immunofluorescence analysis of U2OS cells using EIF3A antibody (CAB0573) at dilution of 1:100. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products

Human eIF3A ELISA Kit

Human eIF3A ELISA Kit

Human eIF3A ELISAEIF3A has been found to play a key role in cellular processes such as gene expression, cell proliferation and apoptosis. EIF3A interacts with certain key proteins such as TPX2,...