Metabolism Antibodies 2

Anti-EIF2B2 Antibody (CAB7027)

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SKU:
CAB7027
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-EIF2B2 Antibody
Antibody SKU:CAB7027
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human EIF2B2 (NP_055054.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse thymus, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human EIF2B2 (NP_055054.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPGS AAKG SELS ERIE SFVE TLKR GGGP RSSE EMAR ETLG LLRQ IITD HRWS NAGE LMEL IRRE GRRM TAAQ PSET TVGN MVRR VLKI IREE YGRL HGRS DESD QQES LHKL LTSG GLNE DFSF HYAQ LQSN IIEA INEL LVEL EGTM ENIA AQAL EHIH SNEV IMTI GFSR TVEA FLKE AARK RKFH VIVA ECAP FCQG HEMA VNLS KAGI ETTV MTDA AIFA VMSR VNKV IIGT KTIL ANGA LRAV TGTH TLAL AAKH HSTP LIVC APMF KLSP QFPN EEDS FHKF VAPE EVLP FTEG DILE KVSV HCPV FDYV PPEL ITLF ISNI GGNA PSYI YRLM SELY HPDD HVL
Gene ID:8892
Uniprot:P49770
Cellular Location:
Calculated MW:38kDa
Observed MW:39kDa
Synonyms:EIF2B2, EIF-2Bbeta, EIF2B
Background:This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation.
UniProt Protein Function:eIF2B-beta: eukaryotic translation initiation factor 2B, subunit 5. A translational regulatory protein that functions in the early steps of protein synthesis by catalyzing the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutation in EIF2B5 causes childhood ataxia with central nervous system hypomyelination/ vanishing white matter leukodystrophy.
UniProt Protein Details:

Protein type:Cell development/differentiation; RNA-binding; Translation; GEFs; GEFs, misc.

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm; cytosol

Molecular Function:protein binding; GTP binding; guanyl-nucleotide exchange factor activity; translation initiation factor activity; S-methyl-5-thioribose-1-phosphate isomerase activity; ATP binding

Biological Process: myelination; response to peptide hormone stimulus; central nervous system development; translation; cellular response to stimulus; cellular protein metabolic process; ovarian follicle development; methionine salvage; response to heat; translational initiation; response to glucose stimulus; gene expression; oligodendrocyte development; regulation of translational initiation; positive regulation of GTPase activity

Disease: Leukoencephalopathy With Vanishing White Matter

NCBI Summary:This gene encodes the beta subunit of eukaryotic initiation factor-2B (EIF2B). EIF2B is involved in protein synthesis and exchanges GDP and GTP for its activation and deactivation. [provided by RefSeq, Aug 2011]
UniProt Code:P49770
NCBI GenInfo Identifier:6226858
NCBI Gene ID:8892
NCBI Accession:P49770.3
UniProt Secondary Accession:P49770,Q99LD9, Q62818,
UniProt Related Accession:P49770
Molecular Weight:351
NCBI Full Name:Translation initiation factor eIF-2B subunit beta
NCBI Synonym Full Names:eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
NCBI Official Symbol:EIF2B2  
NCBI Official Synonym Symbols:EIF2B; EIF-2Bbeta  
NCBI Protein Information:translation initiation factor eIF-2B subunit beta; S20I15; S20III15; eIF-2B GDP-GTP exchange factor subunit beta
UniProt Protein Name:Translation initiation factor eIF-2B subunit beta
UniProt Synonym Protein Names:S20I15; S20III15; eIF-2B GDP-GTP exchange factor subunit beta
Protein Family:Translation initiation factor
UniProt Gene Name:EIF2B2  
UniProt Entry Name:EI2BB_HUMAN
Anti-EIF2B2 Antibody (CAB7027)Western blot analysis of extracts of various cell lines, using EIF2B2 antibody (CAB7027) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-EIF2B2 Antibody (CAB7027)Immunohistochemistry of paraffin-embedded rat lung using EIF2B2 antibody (CAB7027) at dilution of 1:100 (40x lens).
Anti-EIF2B2 Antibody (CAB7027)Immunohistochemistry of paraffin-embedded human tonsil using EIF2B2 antibody (CAB7027) at dilution of 1:100 (40x lens).
Anti-EIF2B2 Antibody (CAB7027)Immunohistochemistry of paraffin-embedded mouse brain using EIF2B2 antibody (CAB7027) at dilution of 1:100 (40x lens).
Anti-EIF2B2 Antibody (CAB7027)Immunofluorescence analysis of C6 cells using EIF2B2 antibody (CAB7027) at dilution of 1:100. Blue: DAPI for nuclear staining.
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