eIF2B epsilon (EIF2B5) Rabbit Monoclonal Antibody (CAB8670)

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SKU:
CAB8670
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
Synonyms:
CLE, CACH, LVWM, EIF-2B, EIF2Bepsilon, eIF2B epsilon (EIF2B5)
Research Use:
Metabolism
Frequently bought together:

Description

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eIF2B epsilon (EIF2B5) Monoclonal Antibody (CAB8670)

The eIF2B epsilon (EIF2B5) Monoclonal Antibody (CAB8670) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. With a high specificity for EIF2B5, this antibody allows for the detection and analysis of EIF2B5 protein levels in various cell types, making it an essential tool for studies in molecular biology and disease research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:eIF2B epsilon (EIF2B5) Monoclonal Antibody
SKU:CAB8670
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC1774
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MAAP VVAP PGVV VSRA NKRS GAGP GGSG GGGA RGAE EEPP PPLQ AVLV ADSF DRRF FPIS KDQP RVLL PLAN VALI DYTL EFLT ATGV QETF VFCC WKAA
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:4000
IHC-P1:50 - 1:200
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CLE, CACH, LVWM, EIF-2B, EIF2Bepsilon, eIF2B epsilon (EIF2B5)
Positive Sample:HeLa, HT-29, U-87MG
Cellular Localization:Cytoplasm, Cytosol, Eukaryotic Translation Initiation Factor 2B Complex, Nucleus.
Calculated MW:80kDa
Observed MW:80kDa

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.

Purification MethodAffinity purification
Gene ID8893
RRIDAB_2863582
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using eIF2B epsilon (EIF2B5) (EIF2B5) Rabbit mAb (CAB8670) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil tissue using eIF2B epsilon (EIF2B5) Rabbit mAb (CAB8670) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon tissue using eIF2B epsilon (EIF2B5) Rabbit mAb (CAB8670) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using eIF2B epsilon (EIF2B5) Rabbit mAb (CAB8670) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
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