Cell Biology Antibodies 8

Prolyl hydroxylase PHD1 (EGLN2) Rabbit Polyclonal Antibody (CAB2252)

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SKU:
CAB2252
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Synonyms:
EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1, Prolyl hydroxylase PHD1 (EGLN2)
Research Use:
Cell Biology
Frequently bought together:

Description

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Prolyl hydroxylase PHD1 (EGLN2) Antibody (CAB2252)

The Prolyl hydroxylase PHD1 (EGLN2) Antibody (CAB2252) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.EGL Nine Homolog 2, also known as EglN2 or PHD1, is a member of the prolyl hydroxylase family and plays a key role in oxygen sensing and response. Dysregulation of EGL Nine Homolog 2 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Prolyl hydroxylase PHD1 (EGLN2) Antibody
SKU:CAB2252
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:AALG GRVL AEVE ALKR GGRL RDGQ LVSQ RAIP PRSI RGDQ IAWV EGHE PGCR SIGA LMAH VDAV IRHC AGRL GSYV INGR TKAM VACY PGNG LGYV RHVD N
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1, Prolyl hydroxylase PHD1 (EGLN2)
Positive Sample:HeLa, A-549, HepG2, U-87MG, MCF-7, Mouse brain, Rat heart
Cellular Localization:Nucleus.
Calculated MW:44kDa
Observed MW:43kDa

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

Purification MethodAffinity purification
Gene ID112398
RRIDAB_2764253
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Prolyl hydroxylase PHD1 (EGLN2) Rabbit pAb (CAB2252) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageImmunoprecipitation analysis of 150 μg extracts of HeLa cells using 3 μg Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (CAB2252). Western blot was performed from the immunoprecipitate using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2)) antibody (CAB2252) at a dilution of 1:1000.
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