Antibody Name:	Anti-EEF1D Antibody
Antibody SKU:	CAB2509
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, PC-3, BT-474, Jurkat, A-549, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATN FLAH EKIW FDKF KYDD AERR FYEQ MNGP VAGA SRQE NGAS VILR DIAR AREN IQKS LAGS SGPG ASSG TSGD HGEL VVRI ASLE VENQ SLRG VVQE LQQA ISKL EARL NVLE KSSP GHRA TAPQ TQHV SPMR QVEP PAKK PATP AEDD EDDD IDLF
Gene ID:	1936
Uniprot:	P29692
Cellular Location:	Nucleus
Calculated MW:	28kDa/29kDa/31kDa/71kDa
Observed MW:	38kDa

Synonyms:

EEF1D, EF-1D, EF1D, FP1047

Background:

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

UniProt Protein Function:	EEF1D: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation elongation; Translation Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: eukaryotic translation elongation factor 1 complex; endoplasmic reticulum; cytoplasm; nucleolus; nucleus; cytosol Molecular Function:signal transducer activity; protein binding; transcription activator binding; DNA binding; translation factor activity, nucleic acid binding; heat shock protein binding; translation elongation factor activity Biological Process: cellular protein metabolic process; translational elongation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; translation; transcription, DNA-dependent; mRNA transcription; gene expression; signal transduction
NCBI Summary:	This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]
UniProt Code:	P29692
NCBI GenInfo Identifier:	20141357
NCBI Gene ID:	1936
NCBI Accession:	P29692.5
UniProt Secondary Accession:	P29692,Q4VBZ6, Q969J1, Q96I38, B4DDU4, D3DWK3, E9PBQ9
UniProt Related Accession:	P29692
Molecular Weight:	281
NCBI Full Name:	Elongation factor 1-delta
NCBI Synonym Full Names:	eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
NCBI Official Symbol:	EEF1D
NCBI Official Synonym Symbols:	EF1D; EF-1D; FP1047
NCBI Protein Information:	elongation factor 1-delta; EF-1-delta; antigen NY-CO-4; guanine nucleotide exchange protein
UniProt Protein Name:	Elongation factor 1-delta
UniProt Synonym Protein Names:	Antigen NY-CO-4
Protein Family:	Elongation factor
UniProt Gene Name:	EEF1D
UniProt Entry Name:	EF1D_HUMAN

	Western blot analysis of extracts of various cell lines, using EEF1D antibody (CAB2509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using EEF1D antibody (CAB2509) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using EEF1D antibody (CAB2509) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using EEF1D antibody (CAB2509). Blue: DAPI for nuclear staining.