Antibody Name:	Anti-EEF1A2 Antibody
Antibody SKU:	CAB7327
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 184-463 of human EEF1A2 (NP_001949.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 184-463 of human EEF1A2 (NP_001949.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NPAT VPFV PISG WHGD NMLE PSPN MPWF KGWK VERK EGNA SGVS LLEA LDTI LPPT RPTD KPLR LPLQ DVYK IGGI GTVP VGRV ETGI LRPG MVVT FAPV NITT EVKS VEMH HEAL SEAL PGDN VGFN VKNV SVKD IRRG NVCG DSKS DPPQ EAAQ FTSQ VIIL NHPG QISA GYSP VIDC HTAH IACK FAEL KEKI DRRS GKKL EDNP KSLK SGDA AIVE MVPG KPMC VESF SQYP PLGR FAVR DMRQ TVAV GVIK NVEK KSGG AGKV TKSA QKAQ KAGK
Gene ID:	1917
Uniprot:	Q05639
Cellular Location:	Nucleus
Calculated MW:	50kDa
Observed MW:	50kDa

Synonyms:

EEF1A2, EEF1AL, EF-1-alpha-2, EF1A, EIEE33, HS1, MRD38, STN, STNL

Background:

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer.

UniProt Protein Function:	EEF1A2: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
UniProt Protein Details:	Protein type:Translation; Translation elongation Chromosomal Location of Human Ortholog: 20q13.3 Cellular Component: cytoplasm; nucleus Molecular Function:GTPase activity; protein binding; protein kinase binding; translation elongation factor activity Disease: Epileptic Encephalopathy, Early Infantile, 33; Mental Retardation, Autosomal Dominant 38
NCBI Summary:	This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014]
UniProt Code:	Q05639
NCBI GenInfo Identifier:	544231
NCBI Gene ID:	1917
NCBI Accession:	Q05639.1
UniProt Secondary Accession:	Q05639,P54266, Q0VGC7, B5BUF3, E1P5J1,
UniProt Related Accession:	Q05639
Molecular Weight:	50,470 Da
NCBI Full Name:	Elongation factor 1-alpha 2
NCBI Synonym Full Names:	eukaryotic translation elongation factor 1 alpha 2
NCBI Official Symbol:	EEF1A2
NCBI Official Synonym Symbols:	HS1; STN; EF1A; STNL; MRD38; EEF1AL; EIEE33; EF-1-alpha-2
NCBI Protein Information:	elongation factor 1-alpha 2
UniProt Protein Name:	Elongation factor 1-alpha 2
UniProt Synonym Protein Names:	Eukaryotic elongation factor 1 A-2; eEF1A-2; Statin-S1
UniProt Gene Name:	EEF1A2
UniProt Entry Name:	EF1A2_HUMAN

	Western blot analysis of extracts of MCF-7 cells, using EEF1A2 antibody (CAB7327). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using EEF1A2 Rabbit pAb (CAB7327) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human brain using EEF1A2 Rabbit pAb (CAB7327) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using EEF1A2 Rabbit pAb (CAB7327) at dilution of 1:100 (40x lens).