Cell Biology Antibodies 1
Anti-EEA1 Antibody (CAB0592)
- SKU:
- CAB0592
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-EEA1 Antibody |
| Antibody SKU: | CAB0592 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1182-1411 of human EEA1 (NP_003557.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HT-1080, LO2, Mouse kidney, Mouse lung, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1182-1411 of human EEA1 (NP_003557.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LKAA VEQE KRNQ QILK DQVK KEEE ELKK EFIE KEAK LHSE IKEK EVGM KKHE ENEA KLTM QITA LNEN LGTV KKEW QSSQ RRVS ELEK QTDD LRGE IAVL EATV QNNQ DERR ALLE RCLK GEGE IEKL QTKV LELQ RKLD NTTA AVQE LGRE NQSL QIKH TQAL NRKW AEDN EVQN CMAC GKGF SVTV RRHH CRQC GNIF CAEC SAKN ALTP SSKK PVRV CDAC FNDL QG |
| Gene ID: | 8411 |
| Uniprot: | Q15075 |
| Cellular Location: | Cytoplasm, Early endosome membrane, Peripheral membrane protein |
| Calculated MW: | 162kDa |
| Observed MW: | 175kDa |
| Synonyms: | EEA1, MST105, MSTP105, ZFYVE2 |
| Background: |
| UniProt Protein Function: | Function: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. |
| UniProt Protein Details: | Subunit structure: Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with RAB31. Interacts with ERBB2. May interact with PLEKHF2. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.17 Ref.18 Subcellular location: Cytoplasm. Early endosome membrane; Peripheral membrane protein Ref.1 Ref.7 Ref.9 Ref.13 Ref.17. Domain: The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns3P-enriched membranes is substantially increased in acidic conditions. Ref.14 Miscellaneous: Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases. Sequence similarities: Contains 1 C2H2-type zinc finger.Contains 1 FYVE-type zinc finger. |
| UniProt Code: | Q15075 |
| NCBI GenInfo Identifier: | 55770888 |
| NCBI Gene ID: | 8411 |
| NCBI Accession: | NP_003557.2 |
| UniProt Secondary Accession: | Q15075,Q14221, |
| UniProt Related Accession: | Q15075 |
| Molecular Weight: | 162,466 Da |
| NCBI Full Name: | early endosome antigen 1 |
| NCBI Synonym Full Names: | early endosome antigen 1 |
| NCBI Official Symbol: | EEA1 |
| NCBI Official Synonym Symbols: | MST105; ZFYVE2; MSTP105 |
| NCBI Protein Information: | early endosome antigen 1; early endosome antigen 1, 162kD; endosome-associated protein p162; early endosome-associated protein; zinc finger FYVE domain-containing protein 2 |
| UniProt Protein Name: | Early endosome antigen 1 |
| UniProt Synonym Protein Names: | Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2 |
| Protein Family: | Early endosome antigen |
| UniProt Gene Name: | EEA1 |
| UniProt Entry Name: | EEA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using EEA1 antibody (CAB0592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded Rat lung using EEA1 antibody (CAB0592) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human mammary cancer using EEA1 antibody (CAB0592) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Mouse kidney using EEA1 antibody (CAB0592) at dilution of 1:100 (40x lens). |
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