Cardiovascular Antibodies

Anti-ECM1 Antibody (CAB16368)

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SKU:
CAB16368
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-ECM1 Antibody
Antibody SKU:CAB16368
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ECM1 (NP_001189787.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, A-375, HepG2
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ECM1 (NP_001189787.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PNEQ KEGT PAPF GDQS HPEP ESWN AAQH CQQD RSQG GWGH RLDG FPPG RPSP DNLN QICL PNRQ HVVY GPWN LPQS SYSH LTRQ GETL NFLE IGYS RCCH C
Gene ID:1893
Uniprot:Q16610
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:19kDa/46kDa/60kDa/63kDa
Observed MW:61kDa
Synonyms:ECM1, URBWD
Background:This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.
UniProt Protein Function:ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space

Molecular Function:protein C-terminus binding; signal transducer activity; protein binding; enzyme binding; protease binding; interleukin-2 receptor binding; laminin binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of peptidase activity; signal transduction; regulation of transcription from RNA polymerase II promoter; negative regulation of bone mineralization; positive regulation of angiogenesis; biomineral formation; negative regulation of cytokine and chemokine mediated signaling pathway; positive regulation of endothelial cell proliferation; angiogenesis; regulation of T-helper 2 type immune response; inflammatory response

Disease: Lipoid Proteinosis Of Urbach And Wiethe

NCBI Summary:This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:Q16610
NCBI GenInfo Identifier:48429255
NCBI Gene ID:1893
NCBI Accession:Q16610.2
UniProt Secondary Accession:Q16610,O43266, Q5T5G4, Q5T5G5, Q5T5G6, Q8IZ60, A8K8S0 B4DW49, B4DY60,
UniProt Related Accession:Q16610
Molecular Weight:Observed MW: 55kDaCalculated MW: 19kDa, 46kDa, 60kDa, 63kDa
NCBI Full Name:Extracellular matrix protein 1
NCBI Synonym Full Names:extracellular matrix protein 1
NCBI Official Symbol:ECM1  
NCBI Official Synonym Symbols:URBWD  
NCBI Protein Information:extracellular matrix protein 1; secretory component p85
UniProt Protein Name:Extracellular matrix protein 1
UniProt Synonym Protein Names:Secretory component p85
Protein Family:Extracellular matrix protein
UniProt Gene Name:ECM1  
UniProt Entry Name:ECM1_HUMAN
Anti-ECM1 Antibody (CAB16368)Western blot analysis of extracts of various cell lines, using ECM1 antibody (CAB16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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