Name: Anti-ECE1 Antibody (CAB5638)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB5638
Availability: OutOfStock

Antibody Name:	Anti-ECE1 Antibody [KO Validated]
Antibody SKU:	CAB5638
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 670-770 of human ECE1 (NP_001388.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 670-770 of human ECE1 (NP_001388.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ADNG GLKA AYRA YQNW VKKN GAEH SLPT LGLT NNQL FFLG FAQV WCSV RTPE SSHE GLIT DPHS PSRF RVIG SLSN SKEF SEHF RCPP GSPM NPPH KCEV W
Gene ID:	1889
Uniprot:	P42892
Cellular Location:	Cell membrane, Single-pass type II membrane protein
Calculated MW:	85kDa/87kDa
Observed MW:	130kDa

Synonyms:	ECE1, ECE
Background:	The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

UniProt Protein Function:	ECE1: Converts big endothelin-1 to endothelin-1. Defects in ECE1 are a cause of Hirschsprung disease cardiac defects and autonomic dysfunction (HSCRCDAD). It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction. Belongs to the peptidase M13 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; Membrane protein, integral; Vesicle; Cell surface; EC 3.4.24.71 Chromosomal Location of Human Ortholog: 1p36.1 Cellular Component: membrane; lysosomal membrane; perinuclear region of cytoplasm; early endosome; plasma membrane; integral to membrane; endosome; vesicle; external side of plasma membrane Molecular Function:protein homodimerization activity; peptide hormone binding; metalloendopeptidase activity; metal ion binding; endopeptidase activity Biological Process: pharyngeal system development; regulation of systemic arterial blood pressure by endothelin; regulation of vasoconstriction; heart development; peptide hormone processing; ear development; hormone catabolic process; protein processing; embryonic digit morphogenesis; proteolysis; positive regulation of receptor recycling Disease: Hirschsprung Disease, Cardiac Defects, And Autonomic Dysfunction; Hypertension, Essential
NCBI Summary:	The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Sep 2009]
UniProt Code:	P42892
NCBI GenInfo Identifier:	1706563
NCBI Gene ID:	1889
NCBI Accession:	P42892.2
UniProt Secondary Accession:	P42892,Q14217, Q17RN5, Q2Z2K8, Q58GE7, Q5THM5, Q5THM7 Q5THM8, Q9UJQ6, Q9UPF4, A8K3P1, B4E291,
UniProt Related Accession:	P42892
Molecular Weight:	87,022 Da
NCBI Full Name:	Endothelin-converting enzyme 1
NCBI Synonym Full Names:	endothelin converting enzyme 1
NCBI Official Symbol:	ECE1
NCBI Official Synonym Symbols:	ECE
NCBI Protein Information:	endothelin-converting enzyme 1; ECE-1
UniProt Protein Name:	Endothelin-converting enzyme 1
Protein Family:	Endothelin-converting enzyme
UniProt Gene Name:	ECE1
UniProt Entry Name:	ECE1_HUMAN

	Western blot analysis of extracts from normal (control) and ECE1 knockout (KO) HeLa cells, using ECE1 antibody (CAB5638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat spleen using ECE1 antibody (CAB5638) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human mammary cancer using ECE1 antibody (CAB5638) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using ECE1 antibody (CAB5638) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using ECE1 Polyclonal Antibody (CAB5638) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.