Name: Anti-EBP Antibody (CAB14845)
Brand: Metabolism Antibodies 1
SKU: CAB14845
Availability: OutOfStock

Antibody Name:	Anti-EBP Antibody
Antibody SKU:	CAB14845
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human EBP (NP_006570.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human EBP (NP_006570.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SGRA AVVP LGTW RRLS LCWF AVCG FIHL VIEG WFVL YYED LLGD QAFL SQLW KEYA KGDS RYIL GDNF TVCM ETIT ACLW GPLS LWVV IAFL RQHP LRFI L
Gene ID:	10682
Uniprot:	Q15125
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	26kDa
Observed MW:	26kDa

Synonyms:

EBP, CDPX2, CHO2, CPX, CPXD, MEND

Background:

The protein encoded by this gene is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. This protein shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of this protein is its high content of aromatic amino acid residues (>23%) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2; also known as Conradi-Hunermann syndrome).

UniProt Protein Function:	EBP: Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers. Defects in EBP are the cause of chondrodysplasia punctata X-linked dominant type 2 (CDPX2); also known as Conradi-Hunermann-Happle syndrome. CDP is a clinically and genetically heterogeneous disorder characterized by punctiform calcification of the bones. The key clinical features of CDPX2 are chondrodysplasia punctata, linear ichthyosis, cataracts and short stature. CDPX2 is a rare disorder of defective cholesterol biosynthesis, biochemically characterized by an increased amount of 8-dehydrocholesterol and cholest-8(9)-en-3-beta-ol in the plasma and tissues. Belongs to the EBP family.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Isomerase; EC 5.3.3.5; Lipid Metabolism - steroid biosynthesis; Endoplasmic reticulum Chromosomal Location of Human Ortholog: Xp11.23-p11.22 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to plasma membrane Molecular Function:drug transporter activity; steroid delta-isomerase activity; transmembrane receptor activity Biological Process: cholesterol biosynthetic process via desmosterol; cholesterol biosynthetic process via lathosterol; cholesterol metabolic process; skeletal development Disease: Chondrodysplasia Punctata 2, X-linked Dominant; Mend Syndrome
NCBI Summary:	The protein encoded by this gene is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. This protein shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of this protein is its high content of aromatic amino acid residues (>23%) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2; also known as Conradi-Hunermann syndrome). [provided by RefSeq, Jul 2008]
UniProt Code:	Q15125
NCBI GenInfo Identifier:	17374795
NCBI Gene ID:	10682
NCBI Accession:	Q15125.3
UniProt Secondary Accession:	Q15125,Q6FGL3, Q6IBI9,
UniProt Related Accession:	Q15125
Molecular Weight:	26,353 Da
NCBI Full Name:	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
NCBI Synonym Full Names:	emopamil binding protein (sterol isomerase)
NCBI Official Symbol:	EBP
NCBI Official Synonym Symbols:	CPX; CHO2; CPXD; MEND; CDPX2
NCBI Protein Information:	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
UniProt Protein Name:	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
UniProt Synonym Protein Names:	Cholestenol Delta-isomerase; Delta(8)-Delta(7) sterol isomerase; D8-D7 sterol isomerase; Emopamil-binding protein
Protein Family:	Ejaculatory bulb-specific protein
UniProt Gene Name:	EBP
UniProt Entry Name:	EBP_HUMAN

Western blot analysis of extracts of various cell lines, using EBP antibody (CAB14845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

EBP Antibody (PACO08982)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)