Epigenetics & Nuclear Signaling Antibodies 2

Anti-EBF3 Antibody (CAB15973)

(No reviews yet) Write a Review
SKU:
CAB15973
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-EBF3 Antibody
Antibody SKU:CAB15973
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EBF3 (NP_001005463.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-251MG, SH-SY5Y
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EBF3 (NP_001005463.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AEAL YSVP RNHN QIPT LGNN PAHT GMMG VNSF SSQL AVNV SETS QAND QVGY SRNT SSVS PRGY VPSS TPQQ SNYN TVST SMNG YGSG AMAS LGVP GSPG F
Gene ID:253738
Uniprot:Q9H4W6
Cellular Location:Nucleus
Calculated MW:60kDa/64kDa
Observed MW:58kDa
Synonyms:EBF3, COE3, EBF-3, HADDS, O/E-2, OE-2
Background:This gene encodes a member of the early B-cell factor (EBF) family of DNA binding transcription factors. EBF proteins are involved in B-cell differentiation, bone development and neurogenesis, and may also function as tumor suppressors. The encoded protein inhibits cell survival through the regulation of genes involved in cell cycle arrest and apoptosis, and aberrant methylation or deletion of this gene may play a role in multiple malignancies including glioblastoma multiforme and gastric carcinoma.
UniProt Protein Function:EBF3: Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Belongs to the COE family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleus

Molecular Function:protein dimerization activity; DNA binding; metal ion binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organismal development

NCBI Summary:This gene encodes a membrane protein that mediates cell adhesion between trophoblastic cells and the epithelial cells of the endometrium. The encoded protein participates in cell signalling during embryo implantation, and may also be involved in cancer formation. This gene is located near several other closely related genes on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]
UniProt Code:Q9H4W6
NCBI GenInfo Identifier:20072474
NCBI Gene ID:7216
NCBI Accession:BC026914
UniProt Secondary Accession:Q9H4W6,Q96SX2, Q9NU89, Q9UPN8, B1AKE9, B1AKF1, F5GY27
UniProt Related Accession:Q12816
Molecular Weight:35,586 Da
NCBI Full Name:Homo sapiens trophinin, mRNA
NCBI Synonym Full Names:trophinin
NCBI Official Symbol:TRO  
NCBI Official Synonym Symbols:MAGED3; MAGE-d3  
NCBI Protein Information:trophinin
UniProt Protein Name:Transcription factor COE3
UniProt Synonym Protein Names:Early B-cell factor 3; EBF-3; Olf-1/EBF-like 2; O/E-2; OE-2
Protein Family:Troponin
UniProt Gene Name:EBF3  
UniProt Entry Name:COE3_HUMAN
Anti-EBF3 Antibody (CAB15973)Western blot analysis of extracts of various cell lines, using EBF3 antibody (CAB15973) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

0 Reviews

View AllClose

Related Products