Antibody Name:	Anti-DUT Antibody
Antibody SKU:	CAB2901
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	LO2, Jurkat, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PAPG PETP AISP SKRA RPAE VGGM QLRF ARLS EHAT APTR GSAR AAGY DLYS AYDY TIPP MEKA VVKT DIQI ALPS GCYG RVAP RSGL AAKH FIDV GAGV IDED YRGN VGVV LFNF GKEK FEVK KGDR IAQL ICER IFYP EIEE VQAL DDTE RGSG GFGS TGKN
Gene ID:	1854
Uniprot:	P33316
Cellular Location:	Mitochondrion, Nucleus
Calculated MW:	17kDa/26kDa
Observed MW:	24kDa

Synonyms:

DUT, dUTPase

Background:

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

UniProt Protein Function:	dUTPase: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Belongs to the dUTPase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.6.1.23; DNA replication; Hydrolase; Motility/polarity/chemotaxis; Nucleotide Metabolism - pyrimidine; Nuclear receptor co-regulator; Mitochondrial Chromosomal Location of Human Ortholog: 15q21.1 Cellular Component: nucleoplasm; mitochondrion; nucleus Molecular Function:protein binding; magnesium ion binding; dUTP diphosphatase activity Biological Process: dUTP catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; DNA replication; dUMP biosynthetic process
NCBI Summary:	This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]
UniProt Code:	P33316
NCBI GenInfo Identifier:	347595814
NCBI Gene ID:	1854
NCBI Accession:	P33316.4
UniProt Secondary Accession:	P33316,O14785, Q16708, Q16860, Q6FHN1, Q6NSA3, Q96Q81 A8K650, B4DPR5,
UniProt Related Accession:	P33316
Molecular Weight:	17,748 Da
NCBI Full Name:	Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
NCBI Synonym Full Names:	deoxyuridine triphosphatase
NCBI Official Symbol:	DUT
NCBI Official Synonym Symbols:	dUTPase
NCBI Protein Information:	deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase; dUTP nucleotidohydrolase
UniProt Protein Name:	Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
UniProt Synonym Protein Names:	dUTP pyrophosphatase
Protein Family:	Deoxyuridine 5'-triphosphate nucleotidohydrolase
UniProt Gene Name:	DUT
UniProt Entry Name:	DUT_HUMAN

	Western blot analysis of extracts of various cell lines, using DUT antibody (CAB2901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded rat spleen using DUT antibody (CAB2901) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using DUT antibody (CAB2901) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using DUT antibody (CAB2901) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using DUT Polyclonal Antibody (CAB2901) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.