Name: Anti-DUSP13 Antibody (CAB6464)
Brand: Cell Biology Antibodies 10
SKU: CAB6464
Availability: OutOfStock

Antibody Name:	Anti-DUSP13 Antibody
Antibody SKU:	CAB6464
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human DUSP13 (NP_057448.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse skeletal muscle, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human DUSP13 (NP_057448.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDSL QKQD LRRP KIHG AVQA SPYQ PPTL ASLQ RLLW VRQA ATLN HIDE VWPS LFLG DAYA ARDK SKLI QLGI THVV NAAA GKFQ VDTG AKFY RGMS LEYY GIEA DDNP FFDL SVYF LPVA RYIR AALS VPQG RVLV HCAM GVSR SATL VLAF LMIC ENMT LVEA IQTV QAHR NICP NSGF LRQL QVLD NRLG RETG RF
Gene ID:	51207
Uniprot:	Q6B8I1/Q9UII6
Cellular Location:	Cytoplasm
Calculated MW:	6kDa/7kDa/9kDa/17kDa/20kDa/22kDa/27kDa
Observed MW:	36kDa

Synonyms:

DUSP13, BEDP, DUSP13A, DUSP13B, MDSP, SKRP4, TMDP

Background:

Members of the protein-tyrosine phosphatase superfamily cooperate with protein kinases to regulate cell proliferation and differentiation. This superfamily is separated into two families based on the substrate that is dephosphorylated. One family, the dual specificity phosphatases (DSPs) acts on both phosphotyrosine and phosphoserine/threonine residues. This gene encodes different but related DSP proteins through the use of non-overlapping open reading frames, alternate splicing, and presumed different transcription promoters. Expression of the distinct proteins from this gene has been found to be tissue specific and the proteins may be involved in postnatal development of specific tissues. A protein encoded by the upstream ORF was found in skeletal muscle, whereas the encoded protein from the downstream ORF was found only in testis. In mouse, a similar pattern of expression was found. Multiple alternatively spliced transcript variants were described, but the full-length sequence of only some were determined.

UniProt Protein Function:	DUSP13 iso5: May be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis. Exhibits intrinsic phosphatase activity towards both phospho- seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro. Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. 5 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:	Protein type:EC 3.1.3.16; Cell cycle regulation; Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, dual-specificity Chromosomal Location of Human Ortholog: 10q22.2 Molecular Function:protein binding Biological Process: meiosis; protein amino acid dephosphorylation; spermatogenesis
NCBI Summary:	Members of the protein-tyrosine phosphatase superfamily cooperate with protein kinases to regulate cell proliferation and differentiation. This superfamily is separated into two families based on the substrate that is dephosphorylated. One family, the dual specificity phosphatases (DSPs) acts on both phosphotyrosine and phosphoserine/threonine residues. This gene encodes different but related DSP proteins through the use of non-overlapping open reading frames, alternate splicing, and presumed different transcription promoters. Expression of the distinct proteins from this gene has been found to be tissue specific and the proteins may be involved in postnatal development of specific tissues. A protein encoded by the upstream ORF was found in skeletal muscle, whereas the encoded protein from the downstream ORF was found only in testis. In mouse, a similar pattern of expression was found. Multiple alternatively spliced transcript variants were described, but the full-length sequence of only some were determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q6B8I1
NCBI GenInfo Identifier:	74748394
NCBI Gene ID:	51207
NCBI Accession:	Q6B8I1.1
UniProt Secondary Accession:	Q6B8I1,Q96J67,
UniProt Related Accession:	Q6B8I1,Q9UII6
Molecular Weight:	17,283 Da
NCBI Full Name:	Dual specificity protein phosphatase 13 isoform A
NCBI Synonym Full Names:	dual specificity phosphatase 13
NCBI Official Symbol:	DUSP13
NCBI Official Synonym Symbols:	BEDP; MDSP; TMDP; SKRP4; DUSP13A; DUSP13B
NCBI Protein Information:	dual specificity protein phosphatase 13
UniProt Protein Name:	Dual specificity protein phosphatase 13 isoform A
UniProt Synonym Protein Names:	Branching-enzyme interacting DSP; Muscle-restricted DSP; MDSP
Protein Family:	Dual specificity protein phosphatase
UniProt Gene Name:	DUSP13
UniProt Entry Name:	DS13A_HUMAN

Western blot analysis of extracts of various cell lines, using DUSP13 antibody (CAB6464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)