Cell Biology Antibodies 14

Anti-DPH1 Antibody (CAB16835)

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SKU:
CAB16835
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DPH1 Antibody
Antibody SKU:CAB16835
Antibody Size:20uL, 50 uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human DPH1 (NP_001374.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human DPH1 (NP_001374.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LQAA AQEL KAEY RVSV PQCK PLSP GEIL GCTS PRLS KEVE AVVY LGDG RFHL ESVM IANP NVPA YRYD PYSK VLSR EHYD HQRM QAAR QEAI ATAR SAKS W
Gene ID:1801
Uniprot:Q9BZG8
Cellular Location:
Calculated MW:
Observed MW:Refer to figures
Synonyms:DPH1, DEDSSH, DPH2L, DPH2L1, OVCA1
Background:The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies.
UniProt Protein Function:DPH1: Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers. Plays a role in embryonic growth, organogenesis and postnatal survival. Belongs to the DPH1/DPH2 family. DPH1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cytosol

Molecular Function:protein binding

Biological Process: peptidyl-diphthamide biosynthetic process from peptidyl-histidine

NCBI Summary:Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9BZG8
NCBI GenInfo Identifier:269849559
NCBI Gene ID:1801
NCBI Accession:Q9BZG8.2
UniProt Secondary Accession:Q9BZG8,Q16439, Q4VBA2, Q9BTW7, Q9UCY0, D3DTI3,
UniProt Related Accession:Q9BZG8
Molecular Weight:33,516 Da
NCBI Full Name:Diphthamide biosynthesis protein 1
NCBI Synonym Full Names:diphthamide biosynthesis 1
NCBI Official Symbol:DPH1  
NCBI Official Synonym Symbols:DPH2L; OVCA1; DEDSSH; DPH2L1  
NCBI Protein Information:diphthamide biosynthesis protein 1
UniProt Protein Name:Diphthamide biosynthesis protein 1
UniProt Synonym Protein Names:DPH1 homolog; HsDph1; Diphthamide biosynthesis protein 2 homolog-like 1; DPH-like 1; DPH2-like 1; Diphthamide biosynthesis protein 2-like; Ovarian cancer-associated gene 1 protein
Protein Family:Diphthamide biosynthesis protein
UniProt Gene Name:DPH1  
UniProt Entry Name:DPH1_HUMAN
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