Cell Biology Antibodies 8

Anti-DOCK2 Antibody (CAB3595)

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SKU:
CAB3595
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-DOCK2 Antibody
Antibody SKU:CAB3595
Antibody Size:20uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1551-1830 of human DOCK2 (NP_004937.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:1000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1551-1830 of human DOCK2 (NP_004937.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FTEE YVRD HPED QDKL THLK DLIA WQIP FLGA GIKI HEKR VSDN LRPF HDRM EECF KNLK MKVE KEYG VREM PDFD DRRV GRPR SMLR SYRQ MSII SLAS MNSD CSTP SKPT SESF DLEL ASPK TPRV EQEE PISP GSTL PEVK LRRS KKRT KRSS VVFA DEKA AAES DLKR LSRK HEFM SDTN LSEH AAIP LKAS VLSQ MSFA SQSM PTIP ALAL SVAG IPGL DEAN TSPR LSQT FLQL SDGD KKTL TRKK VNQF FKTM LASK SAEE GKQI PDSL STDL
Gene ID:1794
Uniprot:Q92608
Cellular Location:Cytoplasm, Endomembrane system, Peripheral membrane protein, cytoskeleton
Calculated MW:38kDa/211kDa
Observed MW:240kDa
Synonyms:DOCK2, IMD40
Background:The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells and is predominantly expressed in peripheral blood leukocytes. The protein is involved in remodeling of the actin cytoskeleton required for lymphocyte migration in response to chemokine signaling. It activates members of the Rho family of GTPases, for example RAC1 and RAC2, by acting as a guanine nucleotide exchange factor (GEF) to exchange bound GDP for free GTP.
UniProt Protein Function:DOCK2: a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis. Plays a role in T cell receptor-induced activation of Rac2 and IL-2 transcription. Interacts with RAC, CRKL, and CD3-zeta. Association with ELMO1 is critical for DOCK2-mediated Rac activation. Two alternatively-spliced isoforms have been described.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; GAPs; GAPs, Rac/Rho; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: cytoskeleton; membrane; endomembrane system; cytosol

Molecular Function:protein binding; Rac guanyl-nucleotide exchange factor activity; T cell receptor binding

Biological Process: alpha-beta T cell proliferation; viral reproduction; formation of immunological synapse; myeloid dendritic cell activation during immune response; small GTPase mediated signal transduction; positive regulation of phagocytosis; establishment of T cell polarity; lipid raft polarization; chemotaxis; actin cytoskeleton organization and biogenesis; negative thymic T cell selection; regulation of defense response to virus by virus; positive thymic T cell selection

Disease: Immunodeficiency 40

NCBI Summary:The DOCK2 gene encodes a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis.[supplied by OMIM]
UniProt Code:Q92608
NCBI GenInfo Identifier:31377468
NCBI Gene ID:1794
NCBI Accession:NP_004937.1
UniProt Secondary Accession:Q92608,Q2M3I0, Q96AK7,
UniProt Related Accession:Q5XG91,Q6ZNS5,Q92608
Molecular Weight:211,948 Da
NCBI Full Name:dedicator of cytokinesis protein 2
NCBI Synonym Full Names:dedicator of cytokinesis 2
NCBI Official Symbol:DOCK2  
NCBI Official Synonym Symbols:FLJ46592; KIAA0209  
NCBI Protein Information:dedicator of cytokinesis protein 2; OTTHUMP00000160994; OTTHUMP00000223166; dedicator of cyto-kinesis 2
UniProt Protein Name:Dedicator of cytokinesis protein 2
Protein Family:Dedicator of cytokinesis protein
UniProt Gene Name:DOCK2  
UniProt Entry Name:DOCK2_HUMAN
Anti-DOCK2 Antibody (CAB3595)Western blot analysis of extracts of Jurkat cells, using DOCK2 antibody (CAB3595). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-DOCK2 Antibody (CAB3595)Immunofluorescence analysis of L929 cells using DOCK2 Rabbit pAb (CAB3595) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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