Developmental Biology

Anti-DNMT3L Antibody (CAB13591)

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SKU:
CAB13591
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-DNMT3L Antibody
Antibody SKU:CAB13591
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 151-386 of human DNMT3L (NP_787063.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, Jurkat, Mouse thymus, Mouse ovary
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 151-386 of human DNMT3L (NP_787063.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SGLL QRRR KWRS QLKA FYDR ESEN PLEM FETV PVWR RQPV RVLS LFED IKKE LTSL GFLE SGSD PGQL KHVV DVTD TVRK DVEE WGPF DLVY GATP PLGH TCDR PPSW YLFQ FHRL LQYA RPKP GSPR PFFW MFVD NLVL NKED LDVA SRFL EMEP VTIP DVHG GSLQ NAVR VWSN IPAI RSRH WALV SEEE LSLL AQNK QSSK LAAK WPTK LVKN CFLP LREY FKYF STEL TSSL
Gene ID:29947
Uniprot:Q9UJW3
Cellular Location:Nucleus
Calculated MW:43kDa
Observed MW:45kDa
Synonyms:DNMT3L
Background:CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Code:Q9UJW3
NCBI GenInfo Identifier:334302913
NCBI Gene ID:29947
NCBI Accession:Q9UJW3.3
Molecular Weight:45kDa
NCBI Full Name:DNA (cytosine-5)-methyltransferase 3-like
UniProt Protein Name:DNA (cytosine-5)-methyltransferase 3-like
Protein Family:DNA (cytosine-5)-methyltransferase
UniProt Gene Name:DNMT3L  
UniProt Entry Name:DNM3L_HUMAN
Anti-DNMT3L Antibody (CAB13591)Western blot analysis of extracts of various cell lines, using DNMT3L antibody (CAB13591) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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