Name: Anti-DNAJC2 Antibody (CAB19954)[KO Validated]
Brand: KO Validated Antibodies 2
SKU: CAB19954
Availability: OutOfStock

Antibody Name:	Anti-DNAJC2 Antibody [KO Validated]
Antibody SKU:	CAB19954
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human DNAJC2.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	293T

Immunogen:	Recombinant protein of human DNAJC2.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	27000
Uniprot:	Q99543
Cellular Location:	Cytoplasm, Nucleus, cytosol
Calculated MW:	65kDa/71kDa
Observed MW:	80KDa

Anti-DNAJC2 Antibody [KO Validated] (CAB19954)

Western blot analysis of extracts from normal (control) and DNAJC2 knockout (KO) 293T cells, using DNAJC2 antibody (CAB19954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Synonyms:

DNAJC2, MPHOSPH11, MPP11, ZRF1, ZUO1

Background:

This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described.

UniProt Protein Function:	DNAJC2: Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb- repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA replication; Chaperone; Cell cycle regulation Chromosomal Location of Human Ortholog: 7q22 Cellular Component: nuclear membrane; cytoplasm; cytosol; nucleus Molecular Function:ubiquitin binding; DNA binding; histone binding; Hsp70 protein binding; chromatin binding Biological Process: 'de novo' cotranslational protein folding; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of cell growth; chromatin modification; DNA replication
NCBI Summary:	This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99543
NCBI GenInfo Identifier:	296439472
NCBI Gene ID:	27000
NCBI Accession:	Q99543.4
UniProt Related Accession:	Q99543
Molecular Weight:
NCBI Full Name:	DnaJ homolog subfamily C member 2
NCBI Synonym Full Names:	DnaJ heat shock protein family (Hsp40) member C2
NCBI Official Symbol:	DNAJC2
NCBI Official Synonym Symbols:	ZRF1; ZUO1; MPP11; MPHOSPH11
NCBI Protein Information:	dnaJ homolog subfamily C member 2
UniProt Protein Name:	DnaJ homolog subfamily C member 2
UniProt Synonym Protein Names:	M-phase phosphoprotein 11; Zuotin-related factor 1DnaJ homolog subfamily C member 2, N-terminally processed
UniProt Gene Name:	DNAJC2
UniProt Entry Name:	DNJC2_HUMAN