Antibody Name:	Anti-DNAJB11 Antibody
Antibody SKU:	CAB12915
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 89-358 of human DNAJB11 (NP_057390.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, 293T, MCF7, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 89-358 of human DNAJB11 (NP_057390.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YGEE GLKD GHQS SHGD IFSH FFGD FGFM FGGT PRQQ DRNI PRGS DIIV DLEV TLEE VYAG NFVE VVRN KPVA RQAP GKRK CNCR QEMR TTQL GPGR FQMT QEVV CDEC PNVK LVNE ERTL EVEI EPGV RDGM EYPF IGEG EPHV DGEP GDLR FRIK VVKH PIFE RRGD DLYT NVTI SLVE SLVG FEMD ITHL DGHK VHIS RDKI TRPG AKLW KKGE GLPN FDNN NIKG SLII TFDV DFPK EQLT EEAR EGIK QLLK QGSV QKVY NGLQ GY
Gene ID:	51726
Uniprot:	Q9UBS4
Cellular Location:	Endoplasmic reticulum lumen
Calculated MW:	40kDa
Observed MW:	40kDa

Synonyms:

DNAJB11, ABBP-2, ABBP2, DJ9, Dj-9, EDJ, ERdj3, ERj3, ERj3p, PRO1080, UNQ537

Background:

This gene encodes a soluble glycoprotein of the endoplasmic reticulum (ER) lumen that functions as a co-chaperone of binding immunoglobulin protein, a 70 kilodalton heat shock protein chaperone required for the proper folding and assembly of proteins in the ER. The encoded protein contains a highly conserved J domain of about 70 amino acids with a characteristic His-Pro-Asp (HPD) motif and may regulate the activity of binding immunoglobulin protein by stimulating ATPase activity.

UniProt Protein Function:	DNAJB11: Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Endoplasmic reticulum; RNA processing; Chaperone Chromosomal Location of Human Ortholog: 3q27.3 Cellular Component: membrane; endoplasmic reticulum; endoplasmic reticulum lumen; nucleus Molecular Function:protein binding; unfolded protein binding Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; protein folding; unfolded protein response; mRNA modification
NCBI Summary:	This gene encodes a soluble glycoprotein of the endoplasmic reticulum (ER) lumen that functions as a co-chaperone of binding immunoglobulin protein, a 70 kilodalton heat shock protein chaperone required for the proper folding and assembly of proteins in the ER. The encoded protein contains a highly conserved J domain of about 70 amino acids with a characteristic His-Pro-Asp (HPD) motif and may regulate the activity of binding immunoglobulin protein by stimulating ATPase activity. [provided by RefSeq, Mar 2014]
UniProt Code:	Q9UBS4
NCBI GenInfo Identifier:	18203497
NCBI Gene ID:	51726
NCBI Accession:	Q9UBS4.1
UniProt Secondary Accession:	Q9UBS4,Q542Y5, Q542Y9, Q6IAQ8, Q96JC6,
UniProt Related Accession:	Q9UBS4
Molecular Weight:	358
NCBI Full Name:	DnaJ homolog subfamily B member 11
NCBI Synonym Full Names:	DnaJ (Hsp40) homolog, subfamily B, member 11
NCBI Official Symbol:	DNAJB11
NCBI Official Synonym Symbols:	DJ9; EDJ; Dj-9; ERj3; ABBP2; ERdj3; ERj3p; ABBP-2; UNQ537; PRO1080
NCBI Protein Information:	dnaJ homolog subfamily B member 11; DnaJ protein 9; APOBEC1-binding protein 2; ER-resident protein ERdj3; PWP1-interacting protein 4; ER-associated DNAJ protein 3; ER-associated Hsp40 co-chaperone; endoplasmic reticulum DNA J domain-containing protein 3
UniProt Protein Name:	DnaJ homolog subfamily B member 11
UniProt Synonym Protein Names:	APOBEC1-binding protein 2; ABBP-2; DnaJ protein homolog 9; ER-associated DNAJ; ER-associated Hsp40 co-chaperone; Endoplasmic reticulum DNA J domain-containing protein 3; ER-resident protein ERdj3; ERdj3; ERj3p; HEDJ; Human DnaJ protein 9; hDj-9; PWP1-interacting protein 4
Protein Family:	DnaJ homolog subfamily
UniProt Gene Name:	DNAJB11
UniProt Entry Name:	DJB11_HUMAN

	Western blot analysis of extracts of various cell lines, using DNAJB11 antibody (CAB12915) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using DNAJB11 antibody (CAB12915) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using DNAJB11 antibody (CAB12915) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using DNAJB11 antibody (CAB12915) at dilution of 1:100 (40x lens).