Antibody Name:	Anti-DNAJA3 Antibody
Antibody SKU:	CAB7030
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_005138.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, Jurkat, 293T, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_005138.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AKGV NKEF TVNI MDTC ERCN GKGN EPGT KVQH CHYC GGSG METI NTGP FVMR STCR RCGG RGSI IISP CVVC RGAG QAKQ KKRV MIPV PAGV EDGQ TVRM PVGK REIF ITFR VQKS PVFR RDGA DIHS DLFI SIAQ ALLG GTAR AQGL YETI NVTI PPGT QTDQ KIRM GGKG IPRI NSYG YGDH YIHI KIRV PKRL TSRQ QSLI LSYA EDET DVEG TVNG VTLT SSGG STMD SSAG SKAR REAG EDEE GFLS KLKK MFTS
Gene ID:	9093
Uniprot:	Q96EY1
Cellular Location:	Cell junction, Cytoplasm, Mitochondrion matrix, Peripheral membrane protein, cytosol, postsynaptic cell membrane, synapse
Calculated MW:	33kDa/49kDa/52kDa
Observed MW:	52kDa

Synonyms:

DNAJA3, HCA57, TID1, hTID-1

Background:

This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.
NCBI Summary:	This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
UniProt Code:	Q96EY1
NCBI GenInfo Identifier:	311033374
NCBI Gene ID:	9093
NCBI Accession:	Q96EY1.2
UniProt Related Accession:	Q96EY1
Molecular Weight:	33kDa; 49kDa; 52kDa
NCBI Full Name:	DnaJ homolog subfamily A member 3, mitochondrial
NCBI Synonym Full Names:	DnaJ heat shock protein family (Hsp40) member A3
NCBI Official Symbol:	DNAJA3
NCBI Official Synonym Symbols:	TID1; HCA57; hTID-1
NCBI Protein Information:	dnaJ homolog subfamily A member 3, mitochondrial
UniProt Protein Name:	DnaJ homolog subfamily A member 3, mitochondrial
UniProt Synonym Protein Names:	DnaJ protein Tid-1; hTid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog
Protein Family:	DnaJ homolog subfamily
UniProt Gene Name:	DNAJA3

	Western blot analysis of extracts of various cell lines, using DNAJA3 antibody (CAB7030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using DNAJA3 Antibody (CAB7030) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using DNAJA3 Antibody (CAB7030) at dilution of 1:100 (40x lens).